Protein Info for BT0307 in Bacteroides thetaiotaomicron VPI-5482

Annotation: phosphofructokinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 69% identical to PFP_PORGN: Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp) from Porphyromonas gingivalis

KEGG orthology group: K00895, pyrophosphate--fructose-6-phosphate 1-phosphotransferase [EC: 2.7.1.90] (inferred from 100% identity to bth:BT_0307)

MetaCyc: 53% identical to diphosphatefructose-6-phosphate 1-phosphotransferase monomer (Borreliella burgdorferi)
Diphosphate--fructose-6-phosphate 1-phosphotransferase. [EC: 2.7.1.90]

Predicted SEED Role

"Pyrophosphate-dependent fructose 6-phosphate-1-kinase (EC 2.7.1.90)" (EC 2.7.1.90)

MetaCyc Pathways

glycolysis IV (9/10 steps found)
pentose phosphate pathway (non-oxidative branch) II (6/6 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)

KEGG Metabolic Maps

Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.90

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8AB05 at UniProt or InterPro

Protein Sequence (548 amino acids)

>BT0307 phosphofructokinase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MTKSALQIARAAYQPKLPKALATGAVKAVAGAATQSVADQEAIKALFPNTYGMPLITFEA
GEAVQLPAMNVGVILSGGQAPGGHNVISGLFDGIKKLNPESKLYGFILGPGGLVDHNYME
LTADIIDEYRNTGGFDIIGSGRTKLETEAQFEKGFEIIKELGIKALVIIGGDDSNTNACV
LAEYYAAKKYGVQVIGCPKTIDGDLKNDMIETSFGFDTACKTYAEVIGNIQRDCNSARKY
WHFIKLMGRSASHIALECALQVQPNVCIVSEEVEEKDMSLDDVVTSIAKVVADRAAQGNN
FGTVLIPEGLVEFIPAMKRLIAELNDFLAANAEEFSQIKKSHQRDYIIRKLSPENSAIYA
SLPEGVARQLTLDRDPHGNVQVSLIETEKLLSEMVATKLAAWKEDGKYVGKFAAQHHFFG
YEGRCAAPSNFDADYCYSLGYTASMLIADGKTGYMSSVRNTTAPAAEWIAGGVPITMMMN
MERRHGEMKPVIQKALVKLDGAPFKAFAAQRDRWAVETDYVYPGPIQYFGPTEVCDQATK
TLQLEQAK