Protein Info for BT0180 in Bacteroides thetaiotaomicron VPI-5482

Annotation: 5-methyltetrahydrofolate-homocysteine methyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 10 to 911 (902 residues), 1339.2 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 14 to 319 (306 residues), 336.3 bits, E=4.5e-104
• PF00809: Pterin_bind
  • amino acids 357 to 595 (239 residues), 208.1 bits, E=3.5e-65
• PF02607: B12-binding_2
  • amino acids 659 to 731 (73 residues), 79 bits, E=4.8e-26
• PF02310: B12-binding
  • amino acids 747 to 843 (97 residues), 78.2 bits, E=9.8e-26

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 100% identity to bth:BT_0180)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (19/25 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• folate transformations II (plants) (8/11 steps found)
• L-methionine salvage from L-homocysteine (2/3 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (8/12 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• folate transformations I (8/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q8ABD0 at UniProt or InterPro

Protein Sequence (915 amino acids)

>BT0180 5-methyltetrahydrofolate-homocysteine methyltransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKTISQIVSERILILDGAMGTMIQQYNLKEEDFRGERFAHIPGQLKGNNDLLCLTRPDV
IQDIHRKYLEAGADIIETNTFSSTTVSMADYHVEEYVREINLAATRLARELADEYTAKSP
DKPRFVAGSVGPTNKTCSMSPDVNNPAFRALSYDELAASYQQQMEAMLEGGVDAILIETI
FDTLNAKAAIFAAGQAMKVTGIEVPVMLSVTVSDIGGRTLSGQTLEAFLASVQHANIFSV
GLNCSFGARQLKPFLEQLASRAPYYISAYPNAGLPNSLGKYDQTPADMAHEVKEYIQEGL
VNIIGGCCGTTDAYIAEYQTLIAGAKPHVPAPKPDCMWLSGLELLEVKPEINFVNIGERC
NVAGSRKFLRLVNEKKYDEALSIARQQVEDGALVIDVNMDDGLLDARTEMTTFLNLIMSE
PEIARVPVMIDSSKWEVIEAGLKCLQGKSIVNSISLKEGEEVFLEHARIIKQYGAATVVM
AFDEKGQADTAARKIEVCERAYRLLVDKVGFNPHDIIFDPNVLAVATGIEEHNNYAVDFI
EATGWIRKNLPGAHVSGGVSNLSFSFRGNNYIREAMHAVFLYHAIQQGMDMGIVNPGTSV
LYSDIPADTLEKIEDVVLNRRPDAAERLIELAEALKETMGGTSGQAAVKQDAWREESVQE
RLKYALMKGIGDYLEQDLAEALPLYDKAVDVIEGPLMDGMNYVGELFGAGKMFLPQVVKT
ARTMKKAVAILQPIIESEKVEGSSSAGKVLLATVKGDVHDIGKNIVAVVMACNGYDIVDL
GVMVPAETIVQRAIEEKVDMIGLSGLITPSLEEMTHVAAELEKAGLDIPLLIGGATTSKM
HTALKIAPVYHAPVVHLKDASQNASVASRLLNSQMKAELINELDAEYQALREKSGLLRRE
TVSLEEAQKNKLNLF