Protein Info for BT0155 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 1 to 353 (353 residues), 338.5 bits, see alignment E=1.9e-105 PF03054: tRNA_Me_trans" amino acids 1 to 196 (196 residues), 202.8 bits, see alignment E=1.2e-63 PF00733: Asn_synthase" amino acids 3 to 71 (69 residues), 21.4 bits, see alignment E=4.5e-08 PF20259: tRNA_Me_trans_M" amino acids 200 to 263 (64 residues), 85 bits, see alignment E=5.3e-28 PF20258: tRNA_Me_trans_C" amino acids 274 to 353 (80 residues), 46.4 bits, see alignment E=1e-15

Best Hits

Swiss-Prot: 100% identical to MNMA1_BACTN: tRNA-specific 2-thiouridylase MnmA 1 (mnmA1) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 100% identity to bth:BT_0155)

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8ABF5 at UniProt or InterPro

Protein Sequence (355 amino acids)

>BT0155 putative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNIAALLSGGVDSSVVVHLLCEQGYKPTLFYIKIGMDGAEYMDCSAEEDIEMSTAIARKY
GLSLEVVDLHKEYWENVAAYAIDKIKKGLTPNPDVMCNKLIKFGCFEQQVGKNFDFTATG
HYATTIRQDGKTWLGTAKDPVKDQTDFLAQIDYLQVSKLMFPIGGLMKQEVREIASRAGL
PSARRKDSQGICFLGKINYNDFVRRFLGEREGAIIELETGKKVGTHRGYWFHTIGQRKGL
GLSGGPWFVIKKDVEENIIYVSHGYGVETQFGSEFRINDFHFITENPWKDAGKEIDITFK
IRHTPEFTKGKLVQEEGGQFRILSSEKLQGIAPGQFGVIYDEEAGICVGSGEITR