Protein Info for BT0124 in Bacteroides thetaiotaomicron VPI-5482

Annotation: NADH:ubiquinone oxidoreductase subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 PF13510: Fer2_4" amino acids 4 to 84 (81 residues), 63.1 bits, see alignment E=7.4e-21 PF10588: NADH-G_4Fe-4S_3" amino acids 92 to 129 (38 residues), 58.4 bits, see alignment 1.4e-19 TIGR02512: [FeFe] hydrogenase, group A" amino acids 147 to 525 (379 residues), 560.2 bits, see alignment E=9.6e-173 PF13237: Fer4_10" amino acids 148 to 209 (62 residues), 24.8 bits, see alignment E=6.1e-09 PF12838: Fer4_7" amino acids 154 to 212 (59 residues), 30.7 bits, see alignment 1.3e-10 PF00037: Fer4" amino acids 196 to 214 (19 residues), 27.8 bits, see alignment (E = 5.6e-10) PF02906: Fe_hyd_lg_C" amino acids 231 to 521 (291 residues), 315.2 bits, see alignment E=1.2e-97 PF02256: Fe_hyd_SSU" amino acids 531 to 585 (55 residues), 86.7 bits, see alignment 3e-28

Best Hits

Swiss-Prot: 65% identical to HNDD_DESFR: NADP-reducing hydrogenase subunit HndD (hndD) from Desulfovibrio fructosivorans

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 100% identity to bth:BT_0124)

Predicted SEED Role

"NADP-reducing [Fe]-hydrogenase, cytoplasmic, alpha subunit (EC 1.12.1.4)" (EC 1.12.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.1.4, 1.6.5.3

Use Curated BLAST to search for 1.12.1.4 or 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8ABI6 at UniProt or InterPro

Protein Sequence (588 amino acids)

>BT0124 NADH:ubiquinone oxidoreductase subunit (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MEEKQITLQIDGHFITVPEGSTILEAACKIGINIPTLCHIDLKGTCIKNNPASCRICVVE
VAGRRNLAPACATRCTEGMVVKTSTLRVMNARKVVAELILSDHPNDCLTCPKCGNCELQT
LALRFNIREMPFNGGELSPRKREVTSSIVRNMDKCIFCRRCESVCNDVQTVGALGAIRRG
FNTTIAPAFDRMMKDSECTYCGQCVAVCPVGALTERDYTNRLLDDLADPDKIVIVQTAPA
VRAALGEEFGLPPGTLVTGKMVYALRELGFDYVFDTDFAADLTIMEEGSEILNRLTRYLD
GDKSVRLPILTSCCPAWVNFFEHHFPDMLDIPSTARSPQQMFGSIAKSYWAEKMGIPREK
LVVVSIMPCLAKKYECDRDEFKVNGVPDVDYSISTRELARLIKRANIGFTLVLDSPFDNP
MGESTGAGVIFGTTGGVMEAALRSVYEIYTGQPLKNVNFEQVRGLSGVRRATIDLNGFEL
KVGIAHGLGNARHLLEDIRNGHNEYHVIEIMACPGGCIGGGGQPLHHGNSDVLYARANAL
YREDANKPLRKSHDNPYIQKLYEEYLGKPLGEKSEMLLHTHYFNKSID