Protein Info for DVUA0011 in Desulfovibrio vulgaris Hildenborough JW710

Name: nifK
Annotation: nitrogenase molybdenum-iron protein beta chain (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR01286: nitrogenase molybdenum-iron protein beta chain" amino acids 11 to 453 (443 residues), 474.5 bits, see alignment E=1.8e-146 PF00148: Oxidored_nitro" amino acids 23 to 443 (421 residues), 466.8 bits, see alignment E=2.9e-144

Best Hits

Swiss-Prot: 58% identical to NIFK_CLOPA: Nitrogenase molybdenum-iron protein beta chain (nifK) from Clostridium pasteurianum

KEGG orthology group: K02591, nitrogenase molybdenum-iron protein beta chain [EC: 1.18.6.1] (inferred from 100% identity to dvl:Dvul_3094)

MetaCyc: 58% identical to [MoFe]-nitrogenase complex nitrogenase component beta subunit (Clostridium pasteurianum)
Nitrogenase. [EC: 1.18.6.1]

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifN" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72WS8 at UniProt or InterPro

Protein Sequence (461 amino acids)

>DVUA0011 nitrogenase molybdenum-iron protein beta chain (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MLKHTPAAYAERSALRVNPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCSYHRSHLTRHF
REPVMASTSSFTEGASVFGGGPNLRQALKTIFQVYDPDMVAVHTTCLSETIGDDIPTITK
VAMEDGTVPQGKKVIHCNTPSYVGSHVTGFSNMVEGMVNYLAAGTGKQAAGQVNVIPGFV
EPSDMRKIKGMLAQMGVDGVVFPDTSDVLDTPQTGEYRMYPAGGTTQADFARSGDSMATL
ALGAWASEAGAKALEKKCGVPYRLLDTPIGVLATDRFLAALQETTGMTVPRSLKDERGKL
VDMMTDYQSLLFGKRVAAWGDPDIVIPLVEFLVSAGMRPTVVVTGTPGKAFERRIAEVME
KAGATWDYEVRAANDLFHLHQRVKAAPVDLLVGNSYGKYIAQAEDTPLVRFGFPILDRVG
HSHFPKVGYMGGMHLLSQVINTLLERKDRDTAPEDTLELVM