Protein Info for DVUA0012 in Desulfovibrio vulgaris Hildenborough JW710

Name: nifD
Annotation: nitrogenase molybdenum-iron protein alpha chain (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 542 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 16 to 530 (515 residues), 672 bits, see alignment E=4.4e-206 TIGR01862: nitrogenase component I, alpha chain" amino acids 31 to 525 (495 residues), 597.4 bits, see alignment E=1.5e-183 PF00148: Oxidored_nitro" amino acids 65 to 518 (454 residues), 418.6 bits, see alignment E=1.2e-129

Best Hits

Swiss-Prot: 56% identical to NIFD_CLOPA: Nitrogenase molybdenum-iron protein alpha chain (nifD) from Clostridium pasteurianum

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 100% identity to dvl:Dvul_3093)

MetaCyc: 56% identical to [MoFe]-nitrogenase complex nitrogenase component alpha subunit (Clostridium pasteurianum)
Nitrogenase. [EC: 1.18.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72WS7 at UniProt or InterPro

Protein Sequence (542 amino acids)

>DVUA0012 nitrogenase molybdenum-iron protein alpha chain (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTTLKTDPGVDLESFVDEVTSLYPPKVAKKRRAHMVVRKDGDPGLAQQIEANTRTVPGII
TQRGCCYAGCKGVVIGPYGDMVHIVHGPVGCSFYAWLTRRNQFRARGEAGKNYLSYCFTT
DMQDEDIIFGGEKKLAQAIREAAAIFRPEAISVHATCPVGLIGDDIQAVARELSKETGIP
VMAFNCEGYKGVSQSAGHHIANNKIFDMALGTREREAGRFSVNVLGEYNIGGDAYEIERV
LEKCGIEVVATFSGNSVYEDMCTAHSANLNLVMCHRSINYLAEMMETKFGIPWMKVNFIG
MRSMSQSLRKIARYFDDAALTARIESVIAEEEARAETALAAYMPGLEGKTAMLFVGGSRA
HHYMHLMRDLGMEPLVAGFEFAHRDDYEGRRVIPTIRVDADSKNIEELKVTADPERYRPR
LDPAYREQLEREGLLSSYEGMWDDMGEGTLVIDDVSHVEIEKLIHRMNPDIICSGIKDKY
VIEKFGVPCKQLHNYDYSGPYAGYEGAVNFARDVYAMIHSPAWKLTRSPFDTGPTLTAGL
ND