Protein Info for DVU0323 in Desulfovibrio vulgaris Hildenborough JW710

Name: folD
Annotation: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 4 to 119 (116 residues), 138.9 bits, E=9.4e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 122 to 282 (161 residues), 216 bits, E=1.8e-68

Best Hits

Swiss-Prot: 100% identical to FOLD_DESVV: Bifunctional protein FolD (folD) from Desulfovibrio vulgaris subsp. vulgaris (strain DP4)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to dvu:DVU0323)

MetaCyc: 61% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (8/12 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• folate transformations III (E. coli) (5/9 steps found)
• folate transformations I (7/13 steps found)
• folate transformations II (plants) (5/11 steps found)
• L-histidine degradation III (1/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See Q72F91 at UniProt or InterPro

Protein Sequence (286 amino acids)

>DVU0323 methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MQLLDGKATAATIREELRAEIAALTPRARRAPGLAVILVGEDPASQVYVRNKERACHDTG
IVSEAFRLAPTTTQEELERLIADLNVRPDIDGILLQLPLPRGLDAQRCLEAIDPAKDVDG
FHPQNMGRLALGLPGFRPCTPAGVMTLLERYDLSPSGRKAVVVGRSNIVGKPLALMLGAP
GKYANATVTVCHSGTPDLAAECRTADFLFLAIGRPRFVTADMVREGAVVVDVGINRTETG
LAGDCDFEGVSRVASAITPVPGGVGPMTIAQLLVNTVQSWKVRCGL