Protein Info for DVU0004 in Desulfovibrio vulgaris Hildenborough JW710

Name: gyrA
Annotation: DNA gyrase, A subunit (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 856 TIGR01063: DNA gyrase, A subunit" amino acids 25 to 823 (799 residues), 1134.3 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 47 to 486 (440 residues), 547 bits, see alignment E=3.4e-168 PF03989: DNA_gyraseA_C" amino acids 519 to 559 (41 residues), 36.4 bits, see alignment 2.8e-13 amino acids 569 to 618 (50 residues), 30.9 bits, see alignment 1.5e-11 amino acids 627 to 664 (38 residues), 28.7 bits, see alignment (E = 7.1e-11) amino acids 673 to 719 (47 residues), 36.2 bits, see alignment 3.3e-13 amino acids 727 to 773 (47 residues), 41.7 bits, see alignment 6.2e-15 amino acids 779 to 823 (45 residues), 37 bits, see alignment 1.8e-13

Best Hits

Swiss-Prot: 75% identical to GYRA_LAWIP: DNA gyrase subunit A (gyrA) from Lawsonia intracellularis (strain PHE/MN1-00)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 100% identity to dvu:DVU0004)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72G56 at UniProt or InterPro

Protein Sequence (856 amino acids)

>DVU0004 DNA gyrase, A subunit (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MERAPHGARRPGSNTTRQAVSEQQQISIEKELRKSYLEYSLSVIIGRAIPDVRDGLKPVH
RRIMFAQHELGNAYNRPHKKSARIVGDVIGKYHPHGDSAVYDAIVRMAQEFSMRDPLVDG
QGNFGSIDGDAAAAMRYTEVRMSRLAGEFIADIEKETVDFRPNYDNTLLEPVVLPTRVPN
LLLNGSTGIAVGMATNIPPHNLGELCNALLHLLDDDACTIDDLMDHVKGPDFPTGGFVYA
GQGLVDAYTTGRGTVKVRGKVEIEERKKGFQAIVIREIPYGLNKSSLVEKIAALVNERRI
DGVADLRDESDRKGIRVVIDLKRGTIPDIVVNALYKYTPLESSYGINMLAVVDNRPQLLD
LKSALSHFLEHRREIIIRRTRYDLRKAEARAHILEGLRIALDNIDEVVALIRASATPQEA
KDRLMQRFGLSEAQSQAILDMRLQRLTNLEHEKLLDEYRELLKKIEYFRSILENIEVLRQ
VMREEFTEIRDTYATKRRTEVLMDELEGIAIEDLIPDDDVVITLSRRGYIKRTTLDNYQQ
QRRGGKGIAGLHTSDDDFVQEFLTTTNHQYLLLFTNHGKMHQLKVHQVAEGSRTAKGVHI
ANLLPLEKDEWVTTVLSVREFAEDRFFLFVTKRGMVKRSSASLYARARKGGLIAVGLRDD
DELIMVREIHNDDHVVLTTGGGMAIRFACPDVRPMGRSATGVKGVALSGDDRVVACAVLP
GDDTSTEIMSISSNGYGKRTRIELYRLQSRGGKGVKNFRVTPKTGDVIGAMPVRDDEALV
LLTSTNKIIRMSVDEVRSVGRATMGVMLVRLDEGAIVVGFDRVDEREVVEESAPAQSSEQ
KTAPEGGDAGTEGTAG