Protein Info for DVU3367 in Desulfovibrio vulgaris Hildenborough JW710

Name: aspS
Annotation: aspartyl-tRNA synthetase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 610 TIGR00459: aspartate--tRNA ligase" amino acids 23 to 599 (577 residues), 775 bits, see alignment E=2.1e-237 PF01336: tRNA_anti-codon" amino acids 40 to 125 (86 residues), 58.9 bits, see alignment E=7.8e-20 PF00152: tRNA-synt_2" amino acids 143 to 575 (433 residues), 406.4 bits, see alignment E=1.7e-125 PF02938: GAD" amino acids 337 to 422 (86 residues), 90.3 bits, see alignment E=1.6e-29

Best Hits

Swiss-Prot: 100% identical to SYDND_DESVH: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to dvl:Dvul_0028)

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q725Q7 at UniProt or InterPro

Protein Sequence (610 amino acids)

>DVU3367 aspartyl-tRNA synthetase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSDQIADIQLEHQQYVAPLGDWQRTHSCCELTAADVGNDVCIMGWVQYRRDHGGLIFVDL
RDRKGLTQVVFSPDFAPEAHKDAHIVRSEYVLAIRGRVRPRPEGMTNPGMKTGEIEVVVS
EWKLLNTSKTPPFLIEDRTEASENLRLAWRYLDLRRPRMARNFMLRHRAAQSARRYLDEL
DFLEIETPYLTKATPEGARDFLVPSRLNHGMFYALPQSPQIFKQLLMVSGMDRYYQIVRC
FRDEDMRADRQLEFTQIDIEMSFVDEERVMSMAEGLMSRVMKDTLGVDVTVPFPRMTYDQ
AMGEYGVDKPDTRFDLRLKDVTDAVRGSEFKLFAKAPLVKAMRVPGGETMTRKEIDEFTE
FVKIYGAQGLAWIKIREGEWQSPIAKFLSEAERAALVAALGLEVGDIVFFQAGEPGMVNA
ALGNLRVKLGQHLGLIPEDTYNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHFDLMT
SDPAAARARAYDMVLNGYELGGGSIRIHSAEVQRRMFAALGLDPQEAEEKFGFLIQALEH
GAPPHGGIAFGMDRLVMLLTGSPSIRDVIAFPKTQKATCLMTQAPDAVSARQLRDLGIRL
RETPQEQKPE