Protein Info for DVU3316 in Desulfovibrio vulgaris Hildenborough JW710

Name: pyrD
Annotation: dihydroorotate dehydrogenase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 12 to 298 (287 residues), 338.1 bits, see alignment E=2.1e-105 PF01180: DHO_dh" amino acids 14 to 288 (275 residues), 241.1 bits, see alignment E=2.9e-75

Best Hits

Swiss-Prot: 73% identical to PYRDB_DESMR: Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit (pyrD) from Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 100% identity to dvl:Dvul_0072)

MetaCyc: 52% identical to dihydroorotate dehydrogenase, catalytic subunit (Lactococcus lactis lactis)
Orotate reductase (NADH). [EC: 1.3.1.14]

Predicted SEED Role

"Dihydroorotate dehydrogenase, catalytic subunit (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1

Use Curated BLAST to search for 1.3.1.14 or 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q725V9 at UniProt or InterPro

Protein Sequence (307 amino acids)

>DVU3316 dihydroorotate dehydrogenase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MDLSVTIPGGLHPLALRNPVLTASGTFGYGVEFEPYGDLTALGGIVVKGLSLKPRLGNPM
PRVAETPCGMLNAVGLQNDGVECFINEKLPRLPWRELPVIANIYACDPGKFGELAAVLAA
EEGVAALEVNISCPNVKEGGILFGQDARQAARVTEAVKMRAGAKPVIVKLSPNVTDIVTM
ARAVEDAGADMLSCINTLSGMGVDIRTRRPLLANVIGGLSGPAIKPVALRCVWQVSRAVS
IPVIGIGGIASAEDVLEFILVGAHAVQVGTANFMRPDFAFRLVDELNELCDRMGITDWDA
FRGSLKA