Protein Info for DVU3014 in Desulfovibrio vulgaris Hildenborough JW710

Annotation: asparagine synthetase, glutamine-hydrolyzing (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 569 (568 residues), 461.4 bits, E=2.5e-142
• PF13522: GATase_6
  • amino acids 46 to 167 (122 residues), 150.8 bits, E=4.4e-48
• PF13537: GATase_7
  • amino acids 52 to 173 (122 residues), 161.1 bits, E=2.2e-51
• PF00733: Asn_synthase
  • amino acids 249 to 633 (385 residues), 311.7 bits, E=2.1e-96

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to dvu:DVU3014)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See Q726U2 at UniProt or InterPro

Protein Sequence (697 amino acids)

>DVU3014 asparagine synthetase, glutamine-hydrolyzing (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MCGIAGIVSPGAPVASATLAAMTESLAHRGPDACGLFIRPSGAGSPHVGLGHRRLSILDL
SDAGAQPMQSEDGAFTLVFNGEIYNYPDLRRDLEAAGHIFRSTSDTEAILRGYMAWGEGV
VARLKGMFAFALWDERKRSLLLARDRFGKKPLHYAVTRQGTLLFASEPKALLAHPEFRPE
PDAASLLHFLRYDYVPAPRSIYAGAAKLPPSHLMVWRDGRLEVRRYASLAFSGATGDRGV
PLREAEAAVDALLDAAVQRRLASDVPLGVFLSGGLDSSTVLAYMTRHIPAHDIRTFAIGF
DEASYDESAHAEAMARHAGSHHTMMRLPAARMLDLLDMVWTTQDEPMADASIIPTLLLCR
FAREHITVALGGDGGDELFAGYDPVLADLPARCLDWLPPAALRLLQGIALRLPTGDANMS
LGFKVQRFLSGLGAREVAARHQTWLGAFDDEGARAMLHPDLRAATRDVSPLDALDMLREE
CRDASRFDAMLHFYQRFYMCGSILTKVDSASMAVSLEVRSPFLDQDLADYVNRLPREHKL
RGLTRKVLLRRLMRSRMPDALLKRPKKGFGLPLARWFRHELRPLVTAMLDPGRVAQGGLL
DPVTVERMVRDHLSGRRDHRKEIWSALCVEAWRERYLHHSRATASDTIEFGTTPHGDGHL
ITAQAVPARSSAAINDSTNDSPSWAASPSRHRCNDSI