Protein Info for DVU2739 in Desulfovibrio vulgaris Hildenborough JW710

Annotation: pyruvate phosphate dikinase, PEP/pyruvate binding domain protein (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 15 to 325 (311 residues), 250.9 bits, E=2.1e-78
• PF00391: PEP-utilizers
  • amino acids 365 to 435 (71 residues), 66.3 bits, 1.6e-22

Best Hits

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to dvl:Dvul_0567)

Predicted SEED Role

"PEP/pyruvate binding domain protein"

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q727W5 at UniProt or InterPro

Protein Sequence (945 amino acids)

>DVU2739 pyruvate phosphate dikinase, PEP/pyruvate binding domain protein (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
VLSLADVTPDMASACGTKSTNLATMRNVLGIPTPPGFVVTARGFERFIEENALGERIAQA
LSRCAATARPGDDPVMLERVSAEIRDMVLHAPVPASLSDAILDAYRALEAATHPGVHVAM
RSSAVGEDTEASFAGQYVTVLNVTAADILTAYREVLASKYSPRAILYRLSYGLEDRDTPM
CVAGITMVRSRASGVIYTVDPSAPDSGSLKVAALLGLGELLAGGEGSADVFHVDRATGDL
RNTELTEKTHRLVCLPDGGISLEAATAEERTRPAIDDAIVQRLHGYGIRLEEYFKCPQDI
EWAVAPDGDLFILQSRPLGLVSAHRPQTAVEYTGHPRLITRGRVASPGAASGVVYNALGN
NPDHVPAGAILVARTASPDYAGLMGRVRGIVTDVGSGASHLASVAREFGIPALFDTRDAT
MRLTHGATVTLDADTTSVYEGTVDELASRTAPARKPFFDSPAHHKLRRVLDLVSPLHLLD
PRSPEFDPAHCASVHDIIRFAHENAMREMFGLCDDSLDVTRAVRLHLGIPIMLYCIDLGG
GLDDGLTSCERVETAHVRSVPMRALLDGLTHPGISWQGGVALNAHSLLSVMASAATADSE
NLGGDSFAVVSRDYMNISAKFGYHYANIDALCSDRPSQNHVVLKFAGGAGSYFGRSLRVS
FLASVLQRLGFGVDIRGDMLEATLTGLDGDAQQELLDQTGRLLAAARLLDMTIGNGADVE
RMVSMFFDGNYDFLGTARPRQIEGFYTDTGNWSMEQCDDVRCIRQDGSEWGRSVGGESSA
VFARMFGPRYQEALDRVETFFHFPLAICQESRMADGSLRLHVRPEGGRMDRAGGLAFGIR
NAGNYFVLRLNAVENNVILFEFVNSRRVRLERVDVRIRSGTWAELGVDICGKTVRCLLDG
VPLFETVLRITPYGYAGLWTKGDSVTLFRDFTVEPALGLAHPIMQ