Protein Info for DVU2568 in Desulfovibrio vulgaris Hildenborough JW710

Name: cpsA
Annotation: peptidase, M20/M25/M40 family (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 TIGR01891: amidohydrolase" amino acids 31 to 391 (361 residues), 372.1 bits, see alignment E=1.6e-115 PF01546: Peptidase_M20" amino acids 88 to 399 (312 residues), 164.8 bits, see alignment E=2.6e-52 PF07687: M20_dimer" amino acids 207 to 297 (91 residues), 40.7 bits, see alignment E=2e-14

Best Hits

Swiss-Prot: 44% identical to YTNL_BACSU: Uncharacterized hydrolase YtnL (ytnL) from Bacillus subtilis (strain 168)

KEGG orthology group: K01436, amidohydrolase [EC: 3.5.1.-] (inferred from 100% identity to dvu:DVU2568)

MetaCyc: 44% identical to N-acetyl-L-cysteine deacetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase homolog (EC 3.5.1.18)" (EC 3.5.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-, 3.5.1.18

Use Curated BLAST to search for 3.5.1.- or 3.5.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q728N5 at UniProt or InterPro

Protein Sequence (412 amino acids)

>DVU2568 peptidase, M20/M25/M40 family (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSGHTAHSGTRHPHTGATVQRLAAEVESHIIAHRRALHAIPETGFEERCTAAYVAETLSG
LGLPVRTGIATTGVTALLDTGLEGPVVMLRADMDALPVTEATGLPFASRHEGRMHACGHD
AHMAMLLGAAEMLSAIVREEPGRLRGKVLFLFQPAEEGPGGAAPMIAEGVLDEPKVDVCL
GAHVWPSLPVGTVGVKPGPLMAAMDRFELAVHGRGGHAATPHLCVDALETATQVVGALQR
VVSRMTDPLEPVILTIGELHAGTAYNVIPGEARMAGTVRTFSPDVRAAWEDRIRTVADGV
CAAMGATATLDFHYCHGPVINTPRVAEVVRRAVVEARGEQAVTTPTPTLGGEDFSCFLER
IPGCFFFVGCGGDVPIHNPRFDLDERCLALGAETFVRAALLFTEGGATGMTA