Protein Info for DVU2533 in Desulfovibrio vulgaris Hildenborough JW710

Name: pheT
Annotation: phenylalanyl-tRNA synthetase, beta subunit (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 797 (797 residues), 900.8 bits, see alignment E=4.4e-275 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 84.8 bits, see alignment E=8.9e-28 PF03483: B3_4" amino acids 215 to 386 (172 residues), 217.8 bits, see alignment E=2e-68 PF03484: B5" amino acids 408 to 472 (65 residues), 65.1 bits, see alignment E=1.3e-21 PF17759: tRNA_synthFbeta" amino acids 492 to 690 (199 residues), 167.2 bits, see alignment E=9.8e-53 PF03147: FDX-ACB" amino acids 708 to 797 (90 residues), 57.8 bits, see alignment E=3.1e-19

Best Hits

Swiss-Prot: 100% identical to SYFB_DESVH: Phenylalanine--tRNA ligase beta subunit (pheT) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to dvu:DVU2533)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q728S0 at UniProt or InterPro

Protein Sequence (798 amino acids)

>DVU2533 phenylalanyl-tRNA synthetase, beta subunit (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MLLSLKWLREFVPFEGTAAELGDRLTMLGLELEEIIRPFDAIEPIVVGHVVSRERHPEAD
KLSVCKVDVGQGEPLDIVCGAPNVAAGQRVPVALVGTTMPGGMVIKKAKLRGQPSHGMIC
SERELGLGEDHDGIMVLPESFRIGARLVDELDLDREVCDIAITPNRADCLSVLGLAREVA
LAFGLPLTMPALDLKEEGADASNALRIDIPDASLCPLFHGRVLEGAAVRKSPAWMRYRLI
AVGVRPISNIVDVTNYILMELGQPLHAYDLDLLAGGRIEVSAAREGERLTTLDGVERVLT
SNDLLIRDGEKPVGLAGVMGGAETEISDKSSRVFLEAAVFRPGTIRKTARRLGLSSEASY
RFERGVDQVVCTYAMNRAAQLIAGLSGATLRPGICHNEPLPWQAPVLRFRRARGEALLGI
SLDETFCRETLERLGCKVDAADAADWKVTAPSHRRDFEREADLIEEVARVRGMDTIEPVL
PKVMRPLDRAGAPESKYSFWLRLKHWAAGLGLNEAINYSFVGQKDLDHLNLAVDGRIPIM
NPLTADQNVLRTELAPGLLQNLRHNIAQGNAGLRLFEVAHIFEADATSDTTARERARLDI
LVYGSRYDSQWPHVEADADYADIKGIVEHCLAFLHLEGATFTLAASHPFLMPCVDVAVQG
RQVGVIGRVRPEIADAYHARKDAWLADLDLDVLRELHDAARIAFRSLPVYPPVRRDITVA
APGSLQVGAVLDHILGLRLPLLCGVELIDVFEPEGKDERNLTFRMTFRHASRTLKDAEVD
KERDKVAHSLVEKLPVRI