Protein Info for DVU2509 in Desulfovibrio vulgaris Hildenborough JW710

Name: murE
Annotation: UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 20 to 483 (464 residues), 490.6 bits, see alignment E=2.6e-151 PF01225: Mur_ligase" amino acids 22 to 95 (74 residues), 38 bits, see alignment E=2.6e-13 PF08245: Mur_ligase_M" amino acids 107 to 311 (205 residues), 165.9 bits, see alignment E=1.7e-52 PF02875: Mur_ligase_C" amino acids 333 to 459 (127 residues), 114.5 bits, see alignment E=9.1e-37

Best Hits

Swiss-Prot: 62% identical to MURE_LAWIP: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Lawsonia intracellularis (strain PHE/MN1-00)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to dvl:Dvul_0736)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q728U3 at UniProt or InterPro

Protein Sequence (486 amino acids)

>DVU2509 UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MKPEQVTFDTLLGRVAATLPQIRTDSRRVGPGDVFVAVPGVAVDGAAFITQAVAQGATMV
VCRADGLQHLPEAAFAVAVHDDPRAALGALGQARYGTASLPFPVVGVTGTNGKTTITYLL
EHLFASAGRRCGVLGTVSYRWPGHVEAAPLTTPDCLQLHEMLGQMAGAGVDVAFMEVSSH
ALDQERVAGIRFAGAVLTNLTQDHLDYHGDMETYFNAKARLFRELPEVGKHCAVNADDPW
GRRLLADLPGAVGFALTGAPVSGTRHLHGEMLRCSTAGVHLRMHFEGRTWEVDSPLVGAH
NASNLLAVQAVGLGLGLSPADFVHLENFTGVPGRLERITNDRGLDVFVDYAHTPDALVNV
LRALRDVGFTRIVTVFGCGGNRDRTKRPLMGAAVAAHADVAVLTSDNPRHEDPLAIMEDV
KPGLAGAREVVAEADRRKAIALALEMLRPGDALLVAGKGHEDYQQVGDRKLPFSDQQTIR
EILGCN