Protein Info for DVU2482 in Desulfovibrio vulgaris Hildenborough JW710
Name: fdnG-2
Annotation: formate dehydrogenase, alpha subunit, selenocysteine-containing (TIGR)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to FDHA_DESGI: Formate dehydrogenase subunit alpha (fdhA) from Desulfovibrio gigas
KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to dvu:DVU2482)MetaCyc: 100% identical to formate dehydrogenase 2 alpha subunit (Desulfovibrio vulgaris)
1.2.1.2-RXN [EC: 1.17.1.9]
Predicted SEED Role
"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)
MetaCyc Pathways
- formate oxidation to CO2 (1/1 steps found)
- purine nucleobases degradation II (anaerobic) (16/24 steps found)
- oxalate degradation VI (1/4 steps found)
- oxalate degradation III (1/5 steps found)
- purine nucleobases degradation I (anaerobic) (5/15 steps found)
- superpathway of C1 compounds oxidation to CO2 (2/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.2
Use Curated BLAST to search for 1.17.1.9 or 1.2.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q728X0 at UniProt or InterPro
Protein Sequence (1003 amino acids)
>DVU2482 formate dehydrogenase, alpha subunit, selenocysteine-containing (TIGR) (Desulfovibrio vulgaris Hildenborough JW710) MRMPRRTFIKLASASAGALAFAGLGQSLAPTVARAAELKIAKAKVTPSVCCFCAVGCGLL VYTDTQTGRSINIEGDPDHPTNEGTLCPKGASIWQTTERSKRVTKVLYRAPGAAAWEEKS WDWALPRIARKIKETRDATFELTNDKGQTVNRTRGIASVGSAAVDNEEGWLMQAMMRALG LVYIESHARIUHSSTVGALAESYGRGAMTNHWIDLKNSDVILIMGSNAAENHPISFKWVT RAQERGATLIHVDPRFTRTSAKADIHAHIRSGTDIAFFGGLIRYIMENDLFFRQYVVDYT NASYIVGADYGFADGLFTGFDAEKGTYNTKKWAFAADENGMTLKDPTLNDPRCVFQLMKA HYARYDRKTVSDVTGISEEQLATLWSTFASTGKPDRAGTILYAMGQCQHTVGVQNIRALS MIQLLLGNIGIAGGGVNALRGESNVQGTTDISLLCDNLSGYLPTPKASWATFDDYVKGTT PVDKDPKSANWWSNRGKYLASYMKSVYPTASHQDGYLWHPKVDDGKITDYSWLQIFERMS KGGFKGAFVWGQNPCAGGANAGKNRKAMETLDWMVVVNLFENESSLFWKGPGVDPAKVKT EVFFLPACMSVEKGGSIANSGRWLQWREPGPKPMGDSRSDGDIVLDLYDEIRKLYREEKG AFPEPVLALDTDYRTDGKYDHHKVAKTLNGKFLTDVTIGDKTYKAGQQVPGFAMLQADGS TTSGCWIFTGCYTDAGNMMARRDRTQTPEQAAIGLFPNWSYAWPANRRILYNRAAVDMTG KPFDPKRAVIAWNGEKWVGDVPDGGWKPGEKLPFIMIREGRGQLFGPGRVDGPFPEHYEP FESPLESHPFSKQRVNPTALAFSHEPKAVRDKRYPFICTTYRVTEQWQSGTMTRNTGWLK EMQPEGFCEISRELAKELGIANGDAVVLESLRGKVQVVAIVTPRLKPFKVMGEVMHEVGI PWQFGWGQHVGKGDSANLLSPSVGDPNTGIPETKVFMVNLRKA