Protein Info for DVU2449 in Desulfovibrio vulgaris Hildenborough JW710

Name: metK
Annotation: S-adenosylmethionine synthetase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00438: S-AdoMet_synt_N
  • amino acids 7 to 105 (99 residues), 150.9 bits, E=2e-48
• TIGR01034: methionine adenosyltransferase
  • amino acids 10 to 388 (379 residues), 575.8 bits, E=2e-177
• PF02772: S-AdoMet_synt_M
  • amino acids 118 to 237 (120 residues), 151.2 bits, E=2.1e-48
• PF02773: S-AdoMet_synt_C
  • amino acids 239 to 377 (139 residues), 214.7 bits, E=6.4e-68

Best Hits

Swiss-Prot: 100% identical to METK_DESVH: S-adenosylmethionine synthase (metK) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K00789, S-adenosylmethionine synthetase [EC: 2.5.1.6] (inferred from 100% identity to dvl:Dvul_0786)

MetaCyc: 58% identical to S-adenosylmethionine synthetase monomer (Bacillus subtilis)
Methionine adenosyltransferase. [EC: 2.5.1.6]

Predicted SEED Role

"S-adenosylmethionine synthetase (EC 2.5.1.6)" in subsystem Methionine Biosynthesis or Methionine Degradation or Quorum Sensing: Autoinducer-2 Synthesis (EC 2.5.1.6)

MetaCyc Pathways

• aspartate superpathway (20/25 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• S-adenosyl-L-methionine biosynthesis (1/1 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• ethene biosynthesis I (plants) (1/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• 2'-deoxymugineic acid phytosiderophore biosynthesis (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (2/7 steps found)
• L-methionine salvage cycle II (plants) (2/11 steps found)
• L-methionine salvage cycle III (2/11 steps found)
• L-methionine salvage cycle I (bacteria and plants) (2/12 steps found)
• superpathway of L-methionine salvage and degradation (4/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.6

Sequence Analysis Tools

See Q729A3 at UniProt or InterPro

Protein Sequence (391 amino acids)

>DVU2449 S-adenosylmethionine synthetase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MIPSKGKYYFTSESVTEGHPDKVADQISDAVLDVLLAQDPNSRVACETLVTTGMAVIAGE
ITTRGYADLPHVVRETIRNIGYNSSEMGFDWQTCAVISSIDKQSADIAQGVDRATNEDQG
AGDQGMMFGFACDETATLMPAPIYWAHQLSQRLTEVRKDGTVDIFRPDGKTQVSFEYVDG
KPVRINNVVVSTQHKDSASQADIIDAVKTHVIRPILEPSGFFDEKACDIFINTTGRFVIG
GPMGDCGLTGRKIIQDTYGGMGHHGGGAFSGKDASKVDRSGAYMARYIAKNVVASGLAPK
CEVQIAYCIGVAEPVSVLVSSQGTASVPDEVLTRAVREVFDLRPFHITRRLDLLRPIYGK
TSCYGHFGRELPEFTWEHTDAAADLRTAAKV