Protein Info for DVU2255 in Desulfovibrio vulgaris Hildenborough JW710

Name: ruvB
Annotation: Holliday junction DNA helicase RuvB (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 PF05496: RuvB_N" amino acids 11 to 169 (159 residues), 252.1 bits, see alignment E=4.8e-79 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 15 to 317 (303 residues), 456.1 bits, see alignment E=2.5e-141 PF00004: AAA" amino acids 46 to 169 (124 residues), 69.6 bits, see alignment E=9.4e-23 PF17864: AAA_lid_4" amino acids 172 to 245 (74 residues), 98.7 bits, see alignment E=3.2e-32 PF05491: WHD_RuvB" amino acids 246 to 317 (72 residues), 89.3 bits, see alignment E=3.1e-29

Best Hits

Swiss-Prot: 100% identical to RUVB_DESVV: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Desulfovibrio vulgaris subsp. vulgaris (strain DP4)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 100% identity to dvl:Dvul_0989)

MetaCyc: 61% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P61531 at UniProt or InterPro

Protein Sequence (320 amino acids)

>DVU2255 Holliday junction DNA helicase RuvB (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTANVCLDESVRPRLLDDFIGQDELRANMRVYLDAARERGQAMDHVLFYGNPGLGKTTLA
QIMAGELGVNLVSTSGPVLERSGDLAAILTNLGRHDLLFVDEIHRMPIAVEEVLYPAMED
FKLDLVIGQGPGARTVKIDVEPFTLVGATTRIGLLSSPLRDRFGIISRLEYYTPADLARI
VARTARIIGANLTEEGAIEIGRRARGTPRIANRLLRRVRDFATVHAGGVISADLASEALG
RMEVDESGLDQMDRKLLEVLIEHYGGGPVGIKTLAVACAEEVRTIEDIYEPYLIQCGFLK
RTPRGRVATAKAYRHLNLLG