Protein Info for DVU2144 in Desulfovibrio vulgaris Hildenborough JW710

Name: gap-2
Annotation: glyceraldehyde 3-phosphate dehydrogenase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00044: Gp_dh_N
  • amino acids 4 to 101 (98 residues), 107.7 bits, E=3.4e-35
• TIGR01534: glyceraldehyde-3-phosphate dehydrogenase, type I
  • amino acids 5 to 324 (320 residues), 400.7 bits, E=2.5e-124
• PF02800: Gp_dh_C
  • amino acids 156 to 312 (157 residues), 203.4 bits, E=1.7e-64

Best Hits

Swiss-Prot: 59% identical to G3P_GEOSE: Glyceraldehyde-3-phosphate dehydrogenase (gap) from Geobacillus stearothermophilus

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 100% identity to dvu:DVU2144)

MetaCyc: 49% identical to chloroplastic glyceraldehyde 3-phosphate dehydrogenase A (Arabidopsis thaliana col)
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating). [EC: 1.2.1.13]; Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). [EC: 1.2.1.13, 1.2.1.12]

Predicted SEED Role

"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12)

MetaCyc Pathways

• homolactic fermentation (11/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• Bifidobacterium shunt (13/15 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis IV (8/10 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• gluconeogenesis I (10/13 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• gluconeogenesis III (9/12 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (9/12 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• heterolactic fermentation (13/18 steps found)
• pyridoxal 5'-phosphate biosynthesis I (5/7 steps found)
• superpathway of glucose and xylose degradation (12/17 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
• Entner-Doudoroff pathway I (6/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• oxygenic photosynthesis (11/17 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (14/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• glycerol degradation to butanol (9/16 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (15/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (14/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.12

Use Curated BLAST to search for 1.2.1.12 or 1.2.1.13

Sequence Analysis Tools

See Q72A53 at UniProt or InterPro

Protein Sequence (333 amino acids)

>DVU2144 glyceraldehyde 3-phosphate dehydrogenase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSLNLGINGFGRIGRYLVRLLAEDNELSIAAINARADNASLAHLFKYDSCHGRFNGEVGH
DADGLIVNGRHIAVTREKINEWRWGELGIDLAVETSGTVKDRAGLAQHIARGAKKCVISA
PGKDADVTIVMGVNHGDYDPAKHDVISAASCTTNCLAPAAKVVHETFGIRHGIMTTVHSY
TMSQRILDGSHKDLRRGRAAALSMIPTTTGAAKATALVYPALAGKLDGMAVRVPTPNVSL
VDLTCELERETTVEEVNAALKAAAAGPMLGNMGFCEEPLVSIDFMGSTFGGVVDAQCTSV
IDKTMLKLIIWYDNEAGFTNQLVRLLRMVGKSL