Protein Info for DVU1585 in Desulfovibrio vulgaris Hildenborough JW710

Updated annotation (from data): Methionine synthase (cobalamin-dependent) (EC 2.1.1.13)
Rationale: Important for fitness in most defined media experiments and cofit with metF (DVU0997)
Original annotation: vitamin B12-dependent methionine synthase family protein (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02574: S-methyl_trans
  • amino acids 14 to 286 (273 residues), 258.4 bits, E=2.4e-80
• PF00809: Pterin_bind
  • amino acids 322 to 544 (223 residues), 74.7 bits, E=1.8e-24
• PF02607: B12-binding_2
  • amino acids 600 to 670 (71 residues), 68.3 bits, E=1.1e-22
• PF02310: B12-binding
  • amino acids 685 to 795 (111 residues), 97.5 bits, E=9.8e-32

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 100% identity to dvu:DVU1585)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (20/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (13/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations III (E. coli) (5/9 steps found)
• L-methionine biosynthesis I (2/5 steps found)
• folate transformations I (7/13 steps found)
• folate transformations II (plants) (5/11 steps found)
• superpathway of L-methionine salvage and degradation (4/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q72BP9 at UniProt or InterPro

Protein Sequence (804 amino acids)

>DVU1585 Methionine synthase (cobalamin-dependent) (EC 2.1.1.13) (Desulfovibrio vulgaris Hildenborough JW710)
MPDFRQALRAGRRLLFDGGLGTMLQARGLPAGVSPEQFCLDRPDVLRGIHADYVRAGADI
LTTNTFGGSRFKLGDGFDVVDFNRRMAAIAREAADASGRQAFVAGSIGPTGHFVKPLGEV
EPAALVAAFREQVRGLVAGGADLLMIETQFDLAEARAAVVAARAECSLPIAVSMTFENGV
SLTGSTPEVFVATMLNLGVDLLGTNCSAGPDQMHDVVASLLASASVPVLVEPNAGLPELI
DGKTVFRLPPAPFAEKTAAFAAMGARVLGGCCGTTPDHIAALRQAVADIPATLPVDSGVG
IVLTTRSHLVRVGGDAPVRIIGERINPTGKKQLIAELQAGDFSLALRFSDEQVEAGAPIL
DVNVGAPMVDEEVLLPDLVQRLITRHGVPLSIDSSNAAAIERALPYCPGSTLVNSISGEP
GRMERLGPLCRDHGAPFILLPLKGRKLPVAATERIAIIEELLVQAEGLGIPRRLVMVDVL
ALAVSSKAEAARQCLETIRWCTANGFATTIGLSNISFGLPARELLNGTFLAMAAGAGLSS
CIAHPGNGRIRETVACADVLLARDANAERFIDAYAAWTPATQGGPVASGPGLASQPPATT
LEEAVVKGDRDGVTAIVESELAAGADPFDLVQTKLIPAINEVGVKYERREYFLPQLIRSA
ETMQTAFRRLQPLLEEMRGAEVRPVIIMATVEGDIHDIGKNIVSLMLGNHGFEVVDLGKD
VKAETIVDAAETHGARIIGLSALMTTTMVRMEDTVKLVRARGLDVKVIVGGAVVTKAFAD
AIGADGYSADAVEAVRLAKSLLAG