Protein Info for DVU1345 in Desulfovibrio vulgaris Hildenborough JW710

Name: proS
Annotation: prolyl-tRNA synthetase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 555 (555 residues), 733.1 bits, E=1.3e-224
• PF00587: tRNA-synt_2b
  • amino acids 95 to 455 (361 residues), 127.6 bits, E=8.6e-41
• PF04073: tRNA_edit
  • amino acids 256 to 367 (112 residues), 95.9 bits, E=2.8e-31
• PF03129: HGTP_anticodon
  • amino acids 472 to 563 (92 residues), 63.4 bits, E=2.6e-21

Best Hits

Swiss-Prot: 100% identical to SYP_DESVH: Proline--tRNA ligase (proS) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to dvu:DVU1345)

MetaCyc: 54% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (1/25 steps found)
• phosphinothricin tripeptide biosynthesis (1/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See Q72CD8 at UniProt or InterPro

Protein Sequence (574 amino acids)

>DVU1345 prolyl-tRNA synthetase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MRWSRCYIPTLKEAPSDAEVVSHKLLVRAGMIRKLTSGIYTFMPMGLRALNKVAAIVREE
MNRAGAQEVLMPMVQPADLWQETGRWEFYGKELLRFRDRNDRDYCLGPTHEEVITDLVRG
EVRSYRQLPINLYQIQTKFRDEIRPRFGLMRGREFVMKDAYSFDRDQSGCDESYKAMYAA
YQRIFSRLGLRFRAVEADSGSIGGSFSHEFMVLADTGEDTLAVCTACEYAANVERAEVTG
TPCTRPAAAALAEVPTPGAHTIEEVSAFLGVPADMLVKTLLFVADGEPVAALVRGDRELN
EVKLKNLLGADSLELATPEQVEAWTGAPVGFAGPVGLHGVKRVFADTELKGDAGWIVGAN
KADTHLREVSLTRDAAIEAYADLRMITASDPCPRCGGAVELPKGIEVGHVFKLGLKYSKS
MNATFLDENGKEQVMVMGCYGIGVSRVVASCIEQNNDGDGIVFPPPIAPYEVALLLLDPK
NEEAAAKAAEIESFLEAEGHDVLLDDRDERPGVKFKDADLIGSPYQLVLGGKGLARGVVE
AKNRRSGEKTELPVEGFAEAFRDWRAGVLKGWGL