Protein Info for DVU1024 in Desulfovibrio vulgaris Hildenborough JW710

Name: rluD/coaE
Annotation: ribosomal large subunit pseudouridine synthase D/dephospho-CoA kinase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 TIGR00005: pseudouridine synthase, RluA family" amino acids 23 to 314 (292 residues), 269.1 bits, see alignment E=5e-84 PF01479: S4" amino acids 27 to 75 (49 residues), 25.4 bits, see alignment 1.4e-09 PF00849: PseudoU_synth_2" amino acids 101 to 255 (155 residues), 118.4 bits, see alignment E=5e-38 PF01121: CoaE" amino acids 325 to 507 (183 residues), 90.9 bits, see alignment E=1.3e-29 TIGR00152: dephospho-CoA kinase" amino acids 326 to 509 (184 residues), 69.8 bits, see alignment E=2.7e-23

Best Hits

KEGG orthology group: K06180, ribosomal large subunit pseudouridine synthase D [EC: 5.4.99.12] (inferred from 100% identity to dvu:DVU1024)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72DA5 at UniProt or InterPro

Protein Sequence (541 amino acids)

>DVU1024 ribosomal large subunit pseudouridine synthase D/dephospho-CoA kinase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MQNTQTALSGKEQAHSLTVPAGMRNERLDRFLGEALSHTDLSREKIKRAIRDGQCTVDGT
VCTTPSTRLRSGQRVELAIAPAATAVEPEEGDLTIIHRDEHLLVIDKPAGLTVHPCPSCP
EGTLVHRLVHHFDALRAQEGFRPGIVHRIDKDTSGLLLVALSEGVRLKLSEAFAERTVEK
EYLALVHGVPPATGEVDAPIGRHPTHKVRMAVVKGGKPARSEWRVLHAAPEGHYALVAVR
IHSGRTHQIRVHMAHAGFPLWGDQLYGPGTPARLREQGYGARQMLHAWHLAFTHPVTGER
LRFTCPPPQDFPDLAIALARRMTRVVITGSPGCGKSALLHCLQDRGVPTWSADAAVAALY
EPGADGWHCLRGRFGDRFVPDDSAPVDRRALLAAMQTEPGLRREVEQMVHPLVRHDMEAF
YTRHAETGAPVAVAEVPLFLEAGWRSGDGSCDVLAGVACPERERMHRLTEIRGWTPETIA
AMTAWQWPDEDKMRACDMLIDNGGSLEDLSGETGRFIAALDTRREAAEARLAEELHDIWK
D