Protein Info for DVU0981 in Desulfovibrio vulgaris Hildenborough JW710

Name: ptsI
Annotation: multiphosphoryl transfer protein, putative (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 TIGR02364: dihydroxyacetone kinase, phosphotransfer subunit" amino acids 2 to 126 (125 residues), 119.5 bits, see alignment E=1.6e-38 PF03610: EIIA-man" amino acids 3 to 118 (116 residues), 83.7 bits, see alignment E=2.8e-27 PF00381: PTS-HPr" amino acids 169 to 245 (77 residues), 67.7 bits, see alignment E=2e-22 TIGR01003: phosphocarrier, HPr family" amino acids 169 to 244 (76 residues), 55.5 bits, see alignment E=6.4e-19 PF05524: PEP-utilisers_N" amino acids 289 to 411 (123 residues), 91.1 bits, see alignment E=1.5e-29 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 289 to 850 (562 residues), 485.5 bits, see alignment E=2.9e-149 PF00391: PEP-utilizers" amino acids 439 to 509 (71 residues), 72.8 bits, see alignment E=3.4e-24 PF02896: PEP-utilizers_C" amino acids 542 to 825 (284 residues), 360.3 bits, see alignment E=1.8e-111

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to dvu:DVU0981)

Predicted SEED Role

"Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), subunit DhaM; DHA-specific IIA component / DHA-specific phosphocarrier protein HPr / DHA-specific EI component" in subsystem Dihydroxyacetone kinases (EC 2.7.1.121)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.121, 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.121 or 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72DE8 at UniProt or InterPro

Protein Sequence (854 amino acids)

>DVU0981 multiphosphoryl transfer protein, putative (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MVGIVVVTHSAVLGQGLRELAEQMTQGRVPLAVAGGIDDPDHPIGTDPVRVMTAIEEVQQ
GDGVLVLMDLGSALMSAETALDLLPPEVASQVRLSAAPLVEGLMAAAVLASTGADLGAVA
EEAQSALAAKRELLGAAAPAAPAMPSAHPEGARDSTVPSSAMPAGEELTLVVPNRLGLHA
RPAARIVTALGPFAADVQLVRGDRVVSARSVNRIATLAVRGGETVTFRAVGGDAALALRA
IEALAAAHFGDAPEAPSKGEAPSPAEAPKDGDMAEAAVSADVQGGGVLRGAAASPGLTVG
NAVWYRPAFDAPDVAPLADDPATEVTRLDAALGAARTELVELERRTVAAAGRKEAEIFAM
HRLLLDDVTIAGAARQRIMDRREAAESAWYEVISDAAATFRQLPEGYMREREADMVDVGA
RVLRLLTGVAAVGPRLGGPSVLLATDLGPSDMATLDPSLVIGIVTVQGGATSHAAILARS
LGIPAVAGLGPALQGVGEGDIVALDGGTGDVWVNPAPGVRAAVEARRDAWLAGREAALAG
AAAPAVTADGRAVHILANIGSSADAGAALKNGAEGVGLFRTEFLFLDRTSPPGEEEQLTA
YVTAAAAMQGRPVVVRTLDIGGDKPVSYLEGFATGEDNPFLGLRGIRFCLERKPLFMTQL
RALLRAAAVHPLKVMFPMVAHPGELAAAKALLDEARATLDAEGMPHGQLDVGIMIEVPAA
VALADQLARDAAFFSIGTNDLAQYVMAADRGNASVAALSDALHPAVLRMVRDTVRAGHAA
GIPVAICGELGGNPEAIPLLVGLELDELSMNGPAIPRAKEVVRGCDTGTCAVLADRAMAL
PDAAAVRRLLQGGS