Protein Info for DVU0701 in Desulfovibrio vulgaris Hildenborough JW710

Name: glcB
Annotation: malate synthase G (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 5 to 725 (721 residues), 1047.8 bits, E=0
• PF20656: MS_N
  • amino acids 22 to 71 (50 residues), 45.9 bits, 6.6e-16
• PF20658: MSG_insertion
  • amino acids 160 to 238 (79 residues), 78.6 bits, E=6.8e-26
• PF01274: MS_TIM-barrel
  • amino acids 340 to 580 (241 residues), 194.4 bits, E=3.4e-61
• PF20659: MS_C
  • amino acids 594 to 676 (83 residues), 80.6 bits, E=2.5e-26

Best Hits

Swiss-Prot: 63% identical to MASZ_ALKEH: Malate synthase G (glcB) from Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 100% identity to dvu:DVU0701)

MetaCyc: 55% identical to malate synthase G (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• glycolate and glyoxylate degradation II (2/2 steps found)
• glyoxylate cycle (4/6 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glyoxylate bypass and TCA (7/12 steps found)
• superpathway of glycol metabolism and degradation (2/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (5/14 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (4/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See Q72E78 at UniProt or InterPro

Protein Sequence (729 amino acids)

>DVU0701 malate synthase G (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTGRIREGGLAVAEPLHRCIEREILPGTGISPSIFWAGLEELVGTLGPRNRELLARRDNM
QAAIDAWHKEHQGRPHDAAAYRRFLEDIRYLLPAPQDLAVETQGVDPEVATIAGPQLVVP
VTNPRYALNAVNARWGSLYDALYGSDVIADSDGAARGAAYNPLRGAKVVAYAAAFLDRTF
PLAGARHADVVRYELTGPPVGRKLSALCADGTVTGLLHPDAFAGYTGGERPTTVLLRNNG
LHVELQFDRRHPVGAAHPAGLKDVVLESAVTTILDCEDSVATVDAEDKALAYRNLLGLFR
GDLETSFDKGGRSVFRRLAADRTYTAPGGAAYALPGRSLMLVRNVGLLMTTDAVLDAAGQ
EIPEGILDGLVTACIGLHDLRGAGRHRNSGAGSVYIVKPKLHGPEEVAFVCEQFRRIEDI
LGMARNTLKMGVMDEERRTTLNLAECIRVASQRIIFINTGFLDRTGDEIHTCMEAGPVVR
KNDMKAQRWMLAYEDWNVDTGLAAGFGGKAQIGKGMWPKPDRLAEMVATKGAHPRAGANC
AWVPSPTAATLHALHYHEVDVFARQRELAGCHRASLDDLLALPLMGEARPSPAEVREELE
NNAQSILGYVVRWVEMGIGCSKVPDIHDVGLMEDRATLRISSQHIANWLRHGICTEDEVR
DVMLRMATVVDRQNKDEPGYRPMSADPGSSVAFRAACDLVFEGARQPNGYTEPILHARRR
EVKARSLTR