Protein Info for DVU0650 in Desulfovibrio vulgaris Hildenborough JW710

Annotation: chelatase, putative (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to CBIKP_DESVH: Sirohydrochlorin cobaltochelatase CbiKP (cbiKp) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K02190, sirohydrochlorin cobaltochelatase [EC: 4.99.1.3] (inferred from 100% identity to dvu:DVU0650)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis or Experimental tye or Heme and Siroheme Biosynthesis (EC 4.99.1.3, EC 4.99.1.4)

MetaCyc Pathways

adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
siroheme biosynthesis (4/4 steps found)
factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3, 4.99.1.4

Use Curated BLAST to search for 4.99.1.3 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72EC8 at UniProt or InterPro

Protein Sequence (297 amino acids)

>DVU0650 chelatase, putative (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSRHPMVTRLLCLVFSCLIILACSPAFAGHGAPKAQKTGILLVAFGTSVEEARPALDKMG
DRVRAAHPDIPVRWAYTAKMIRAKLRAEGIAAPSPAEALAGMAEEGFTHVAVQSLHTIPG
EEFHGLLETAHAFQGLPKGLTRVSVGLPLIGTTADAEAVAEALVASLPADRKPGEPVVFM
GHGTPHPADICYPGLQYYLWRLDPDLLVGTVEGSPSFDNVMAELDVRKAKRVWLMPLMAV
AGDHARNDMAGDEDDSWTSQLARRGIEAKPVLHGTAESDAVAAIWLRHLDDALARLN