Protein Info for SO4731 in Shewanella oneidensis MR-1

Name: add
Updated annotation (from data): Adenosine deaminase (EC 3.5.4.4)
Rationale: Specifically important for utilizing Adenosine. Automated validation from mutant phenotype: the predicted function (ADENODEAMIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: adenosine deaminase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00962: A_deaminase
  • amino acids 7 to 327 (321 residues), 362.9 bits, E=1.5e-112
• TIGR01430: adenosine deaminase
  • amino acids 7 to 328 (322 residues), 350.4 bits, E=4.3e-109
• PF19326: AMP_deaminase
  • amino acids 192 to 324 (133 residues), 36.8 bits, E=1.7e-13

Best Hits

Swiss-Prot: 100% identical to ADD_SHEON: Adenosine deaminase (add) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01488, adenosine deaminase [EC: 3.5.4.4] (inferred from 100% identity to son:SO_4731)

MetaCyc: 62% identical to adenosine deaminase (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; 3.5.4.4 [EC: 3.5.4.4]

Predicted SEED Role

"Adenosine deaminase (EC 3.5.4.4)" in subsystem Purine conversions (EC 3.5.4.4)

MetaCyc Pathways

• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• adenosine nucleotides degradation II (3/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.4

Sequence Analysis Tools

See Q8E8D4 at UniProt or InterPro

Protein Sequence (331 amino acids)

>SO4731 Adenosine deaminase (EC 3.5.4.4) (Shewanella oneidensis MR-1)
MINTSIPLVDLHRHLDGNVRVNTIWELGHQHGIALPADSLETLAPFVQIQGKETSLVAFL
KKLDWMVAVLADLDAVKRIAYENVADAALSGLDYAELRFSPYYMAMNHKLPIEGVVEAVI
DGVKAGLKDYQVNINLIGIMSRSFGQPACTQELEGLLAHKQHLVAMDLAGDELGFPGELF
NEHFKRVRDAGLAITAHAGEAAGSQSMWQAIQELGATRIGHGVNAIHDPKLMEYLAKHRI
GIESCPTSNLHTSTVSSYAEHPFRTFMDAGVLIGLNTDDPGVSAIDIKHEYRIAKFELGL
SDAELAQVQRNGVEMAFLSESERKALYAAKA