Protein Info for SO4602 in Shewanella oneidensis MR-1

Name: plsB
Annotation: glycerol-3-phosphate acyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF27464: PlsB_N
  • amino acids 8 to 210 (203 residues), 241.2 bits, E=1.2e-75
• TIGR03703: glycerol-3-phosphate O-acyltransferase
  • amino acids 12 to 797 (786 residues), 1176.7 bits, E=0
• PF01553: Acyltransferase
  • amino acids 285 to 427 (143 residues), 93.8 bits, E=1.2e-30
• PF19277: GPAT_C
  • amino acids 438 to 770 (333 residues), 344 bits, E=1.6e-106

Best Hits

Swiss-Prot: 100% identical to PLSB_SHEON: Glycerol-3-phosphate acyltransferase (plsB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 100% identity to son:SO_4602)

MetaCyc: 52% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (8/13 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (3/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

See Q8E8Q9 at UniProt or InterPro

Protein Sequence (809 amino acids)

>SO4602 glycerol-3-phosphate acyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MPKQDSLWLKSLRWIQKYLVHTIVVPQDPFADLNLDASRPLAYVMKTESLSDIAALSEIT
AKLGLPSPYEPLVVNGVVAPRVVCLEGRKPLFGDRASNEPFLECFMRLLAVHKEKPELDI
QLVPVSLYWGRTPGKEDDTMKAAVLERENPTWLRKCLMILFLGRHNFVQFSNAVSLRYMA
DEHGTDMGIAHKLARVARVHFRRQRKVMTGPVLPNRQALFDSLLKSESLRKAIQEEAASK
KISETQARETAIEYLDEIAANYSDSLVRIAERFLTWLWNKLYSGINIKGAEQIRQLHHDG
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPLFRRGGAFFIRRSFN
GNKLYTAVFREYLDQLFAKGYSVEYFSEGGRSRTGRLLAPKTGMIAMTINSVLRGIERPV
TLVPVYLGYDHVMEVATYHKELSGKKKQKESVWQVFGAIRKLGNFGQGYVNFGEPITLQN
FLNETAPNWRSEVADDPEQKPTWLTPAVNVLANRVMTRINDAAAASSITLTSLVLLASEQ
NALERCLLERQLDLYLTLLKRVPYTSFTSVAEGDGKHLVQQGLELNKFSISADPLGEIVS
IDDKQAISMTYYRNNIIHLFIIPSLIASCLINNKQISRAQIFGVVNDFYPLLKAELFMGI
KDLPSYVDQVLDLFIEQGLVEETELLSVATERASQMLLLAGSVNETLQRYAIIFNLLAHR
PKMERSDLESESHLLAQRLGALHGITAPEFYDKKLYNTLSVKLKELGYFSGKEDKSDVER
IREQANNLLRASVRQTIVASVTAEQSFNG