Protein Info for SO4587 in Shewanella oneidensis MR-1

Annotation: conserved hypothetical protein TIGR00095 (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 190 PF03602: Cons_hypoth95" amino acids 1 to 178 (178 residues), 197.5 bits, see alignment E=1.6e-62 TIGR00095: 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD" amino acids 2 to 178 (177 residues), 189.7 bits, see alignment E=2.1e-60 PF05175: MTS" amino acids 28 to 127 (100 residues), 31 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 57% identical to RSMD_ECOLI: Ribosomal RNA small subunit methyltransferase D (rsmD) from Escherichia coli (strain K12)

KEGG orthology group: K08316, ribosomal RNA small subunit methyltransferase D [EC: 2.1.1.171] (inferred from 100% identity to son:SO_4587)

MetaCyc: 57% identical to 16S rRNA m2G966 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6515 [EC: 2.1.1.171]

Predicted SEED Role

"16S rRNA (guanine(966)-N(2))-methyltransferase (EC 2.1.1.171)" (EC 2.1.1.171)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.171

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E8S4 at UniProt or InterPro

Protein Sequence (190 amino acids)

>SO4587 conserved hypothetical protein TIGR00095 (NCBI ptt file) (Shewanella oneidensis MR-1)
MRIIAGQWRSRKLPIHDLDGLRPTTDRVRETLFNWLANDIAHARVLDCFGGSGALALESL
SRYASYAKIIELQRTAAMQLKANLNTLKCDKAEVINADTLVVLQNGCDQGFDVVFIDPPF
RKGLAEKTIQLLDSKGWLNDGALIYVEIEAELTQMAIPASWQPLKDKTTGQVSYRLYQYQ
AEENPANTEE