Protein Info for SO4583 in Shewanella oneidensis MR-1

Name: rpoH
Annotation: RNA polymerase sigma-32 factor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 TIGR02392: alternative sigma factor RpoH" amino acids 18 to 284 (267 residues), 426.2 bits, see alignment E=6e-132 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 52 to 282 (231 residues), 116.4 bits, see alignment E=9.8e-38 PF04542: Sigma70_r2" amino acids 56 to 125 (70 residues), 66.4 bits, see alignment E=1.6e-22 PF04545: Sigma70_r4" amino acids 230 to 280 (51 residues), 54.6 bits, see alignment 6e-19

Best Hits

Swiss-Prot: 71% identical to RPOH_PROMI: RNA polymerase sigma factor RpoH (rpoH) from Proteus mirabilis

KEGG orthology group: K03089, RNA polymerase sigma-32 factor (inferred from 100% identity to son:SO_4583)

MetaCyc: 69% identical to RNA polymerase sigma factor RpoH (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor RpoH" in subsystem Heat shock dnaK gene cluster extended or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E8S8 at UniProt or InterPro

Protein Sequence (285 amino acids)

>SO4583 RNA polymerase sigma-32 factor (NCBI ptt file) (Shewanella oneidensis MR-1)
MTFQTQSMALTVPQGSSSLEAYIHSVTSMNMLDAETEYELAKRLQETGDLQAAKQLIMSH
LRFVVHVAKGYAGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
LKNWRIVKVATTKAQRKLFFNIRKAKTRLGWFSDDEVTMVAENLGVSKADVTEMESRMAA
QDPAFDLSSDNDDEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQH
ILRARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA