Protein Info for SO4513 in Shewanella oneidensis MR-1

Annotation: formate dehydrogenase, alpha subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 signal peptide" amino acids 1 to 53 (53 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 20 to 42 (23 residues), 18.4 bits, see alignment (E = 1e-07) PF04879: Molybdop_Fe4S4" amino acids 64 to 112 (49 residues), 41.9 bits, see alignment 1.2e-14 PF00384: Molybdopterin" amino acids 118 to 569 (452 residues), 183.2 bits, see alignment E=1.2e-57 PF01568: Molydop_binding" amino acids 816 to 941 (126 residues), 67 bits, see alignment E=2.2e-22

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 77% identity to fbl:Fbal_3586)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E8Y7 at UniProt or InterPro

Protein Sequence (950 amino acids)

>SO4513 formate dehydrogenase, alpha subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MKLTRKSDVAQVANKPTLGISRRQFMKQAGIATGGIAAASLMGTGMMRRAEAKDVPYDAP
IEIKRTICSACAVGCGLYAEVQNGVWTGQEPAFDHPFNAGGHCAKGAALREHGHGEKRLK
YPMKLVDGKWKKISWEDAINEVGDQMLNIRKESGPDSVYFMGSAKFSNEGCYAYRKLAAM
WGTNNVDHSARICHSTTVAGVANTWGYGAQTNSFNDIQNANAIFLIGANPAEAHPVSMQH
ILIAKEKNNAKIIVVDPRFSRTAAHSDLHCAIRPGTDIPFIYGMLWHIFENGWEDKTFIQ
HRVFEMETIRAEVKKFPPKEVANITGVSEEVVYQAAKLMAENRPGTVIWCMGGTQHHVGN
ANTRAYCILQLALGNMGVSGGGTNIFRGHDNVQAATDLGLLFDNLPGYYGLTTAAWEHWT
NVWELDMEWMKSRFDHGTYLGREPMTTPGMPCSRWFDGVLLEKDKLAQKDNIRMAFFWGQ
SVNTGTRQRDVRDALDKLDTVVVVDPFPTIAGIMHSRKNGVYLLPACTQFEASGAVSNSG
RSIQWREQVIQPLFESKNDIEIMYMLAKKVGISEQWAKRWNIAGNMPVVEDISREINRGM
WTIGYTGQSPERIKQHTQNWGTFSNKTLEAAGGPCKGETYGLPWPCWGTPEAKHPGTQIL
YNQDKHVKDGGGNFRARYGVEYNGKNLLAEGTFSKGAEIQDGYPEFSDKLLKQLGWWDDL
TAEEKAAAEGKNWKTDLSGGIVRVAIKHGCIPFGNAKARCIVWTFPDQAPVHREPIYTAR
RDLVAKYPTYDDMQVHRLPTLYKTLQENDLSGKYPLVMTSGRLVEYEGGGEESRCNPWLA
ELQQEMFIEISPADAADRAIRNGEFVWVEGAEGGRIKVQAMVTPRVAPGVTFMPYHFAGV
MHGESLAANYPEGTVPYVIGESCNTALTYGYDPVTQMQETKASLCQIVKA