Protein Info for SO4344 in Shewanella oneidensis MR-1

Name: ilvA
Annotation: threonine dehydratase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01124: threonine ammonia-lyase, biosynthetic
  • amino acids 33 to 534 (502 residues), 765.2 bits, E=1.4e-234
• PF00291: PALP
  • amino acids 47 to 337 (291 residues), 266.6 bits, E=2.9e-83
• PF00585: Thr_dehydrat_C
  • amino acids 350 to 440 (91 residues), 79.6 bits, E=1.2e-26
  • amino acids 453 to 534 (82 residues), 84.4 bits, E=4e-28

Best Hits

Swiss-Prot: 60% identical to ILVA_PASMU: L-threonine dehydratase biosynthetic IlvA (ilvA) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 100% identity to son:SO_4344)

MetaCyc: 61% identical to threonine deaminase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]

Predicted SEED Role

"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-threonine degradation I (6/6 steps found)
• L-methionine degradation II (3/3 steps found)
• L-threonine degradation V (1/2 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.19

Sequence Analysis Tools

See Q8E9E0 at UniProt or InterPro

Protein Sequence (545 amino acids)

>SO4344 threonine dehydratase (NCBI ptt file) (Shewanella oneidensis MR-1)
MLSLASSQTEQAESQPAQASPASVEKSQLAQHYLQKILLSSVYDVAKVTPLSSLNKLSAR
LGCQVFLKREDMQPVHSFKLRGAYNRIAQLSQAECQRGVVCASAGNHAQGVAMSAASRGV
DAVIVMPETTPDIKVDAVRRLGGNVVLHGQAFDQANGFAMTMAQQEGRVYIAPFDDEAVI
AGQGTIAQEMLQQQRDLEVIFVPVGGGGLIAGIAAYYKAVMPQVKIVGVEPEDAACLKAA
MEAGEPVTLSQVGLFADGVAVKRIGTEPFRVAKLCVDAVVTVTSDEICAAVKDIFEDTRA
IAEPAGALSLAGLKKYVSTNATGESGRGEKVAAILSGANVNFHSLRYVSERCELGEQKEA
VLAVKVPERPGSFLRFCELLEKRVMTEFNYRFSSRDMAVVFAGIRLTKGHGELEQIINTL
EDNGFEVQDLSGDETAKLHVRYMVGGHPPEPLEERLFSFEFPEHPGALLKFLTTLQSKWN
ISLFHYRNHGAAFGRVLAGFEVPASDALPFQQFLTELGFVYQEETQSPAYQLFLNAGKGK
NNLPV