Protein Info for SO4218 in Shewanella oneidensis MR-1

Name: murC
Annotation: UDP-N-acetylmuramate--alanine ligase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 22 to 471 (450 residues), 596 bits, E=2.8e-183
• PF01225: Mur_ligase
  • amino acids 22 to 120 (99 residues), 107.9 bits, E=4.2e-35
• PF08245: Mur_ligase_M
  • amino acids 125 to 305 (181 residues), 102.9 bits, E=3.6e-33
• PF02875: Mur_ligase_C
  • amino acids 327 to 462 (136 residues), 58.3 bits, E=2.2e-19

Best Hits

Swiss-Prot: 100% identical to MURC_SHEON: UDP-N-acetylmuramate--L-alanine ligase (murC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 100% identity to son:SO_4218)

MetaCyc: 65% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See Q8E9P8 at UniProt or InterPro

Protein Sequence (488 amino acids)

>SO4218 UDP-N-acetylmuramate--alanine ligase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTKTERYLQLRSMIPEMRRIKRIHFVGIGGAGMGGIAEVLVNEGYVVSGSDIAQNAVTDR
LCLLGAKIHIGHGADNVQQADVVVVSTAINPQNPEIIAAKELRIPIVRRAEMLAELMRYR
HGVAIAGTHGKTTTTSLIASLYGQAGRDPTFVIGGLLNSAGTNARLGTSRYLIAEADESD
ASFLHLQPMVSVVTNIEADHMDTYGGDFEKLKSTFVDFLHNLPFYGVAVVCIDDPVVREI
MPRISRHIVTYGFRDDADVQALNFSQQGHQCRFTVRRKGKEDLDLLLNLPGQHNVLNALA
AIAVATEDEIDDSAIIQALAEFQGIGRRFQHLGKFATPKGEVMLVDDYGHHPSEVAATIK
AARAGWPEKRLVMAYQPHRYTRTRDLYEDFIEVLSQVDCLLLLDVYSAGEAPIPGADGRA
LCRSIRLRGQLDPIFIASPEQLAEVLPDVLQEGDLLLTQGAGNIGALSRKLAASELGFST
GATTEVKP