Protein Info for SO4122 in Shewanella oneidensis MR-1

Name: priA
Annotation: primosomal protein N' (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 PF17764: PriA_3primeBD" amino acids 6 to 109 (104 residues), 92.9 bits, see alignment E=3.1e-30 PF21213: WHD_PriA" amino acids 124 to 181 (58 residues), 37.1 bits, see alignment 9.1e-13 PF04851: ResIII" amino acids 208 to 341 (134 residues), 52.3 bits, see alignment E=2.3e-17 PF00270: DEAD" amino acids 212 to 373 (162 residues), 67.9 bits, see alignment E=3.3e-22 TIGR00595: primosomal protein N'" amino acids 229 to 733 (505 residues), 588.7 bits, see alignment E=4.7e-181 PF18319: Zn_ribbon_PriA" amino acids 453 to 477 (25 residues), 32.6 bits, see alignment (E = 2.2e-11) PF00271: Helicase_C" amino acids 503 to 597 (95 residues), 31.2 bits, see alignment E=8.2e-11 PF18074: PriA_C" amino acids 642 to 733 (92 residues), 64.4 bits, see alignment E=5.1e-21

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 100% identity to son:SO_4122)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E9Y8 at UniProt or InterPro

Protein Sequence (737 amino acids)

>SO4122 primosomal protein N' (NCBI ptt file) (Shewanella oneidensis MR-1)
MSLFVEVALPVPMRQNFTYRVKTNEQNDDSPQAKPQIGVRVKVPFGRQQLIGLVTAITDS
CDLAPTQLKSVIEFIDDAPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAP
QKIQYWQITTIGSNIAPETLKRAPAQKKLLEHLKQTSLTQEEVISLELNKTALKTLEEKG
WIAQHEKIAQLNLDWRSQLELDETPHRLNKEQAVAVTVLTQQQGYHCTLLEGITGSGKTE
VYLALLEHILKQGKQALILVPEIGLTPQTINRFKRRFRVNVAVLHSGLTDNQRLEAWRQA
RCGQAAIIIGTRSALFTPMAFPGVIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPV
VLGSATPSLESLQNALSGRYHHLQLGERAGNAKKVRQGIIDIKNLPLKAGMSAPLINEIR
SHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEIRCHHCGNQYAI
PRQCHNCGSTMLMGQGIGTEQLAEALQLEFPKYPVVRIDRDTTRLKGSLESHLSAIHKGE
YKILVGTQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRANKLG
TVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQMALLPPAWHMVLVRAEAHSALDADNF
LNSFASLLPQDKEFEIIGPMPAPLDRKAGKFRRQLMFQAKHRHRLQQEFERIYPLVEQLP
EAKRCRWSLDRDPQDLL