Protein Info for SO3789 in Shewanella oneidensis MR-1

Annotation: aminotransferase, class V (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 58 to 455 (398 residues), 530.5 bits, E=1.3e-163
• PF00266: Aminotran_5
  • amino acids 77 to 444 (368 residues), 470.7 bits, E=3.4e-145
• PF00155: Aminotran_1_2
  • amino acids 84 to 296 (213 residues), 40.7 bits, E=1.8e-14

Best Hits

Swiss-Prot: 50% identical to SUFS_CITK8: Cysteine desulfurase (sufS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 100% identity to son:SO_3789)

MetaCyc: 48% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• superpathway of L-alanine biosynthesis (4/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• molybdopterin biosynthesis (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (6/11 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Sequence Analysis Tools

See Q8EAV2 at UniProt or InterPro

Protein Sequence (456 amino acids)

>SO3789 aminotransferase, class V (NCBI ptt file) (Shewanella oneidensis MR-1)
MTHTASTNTTLPNAELAPQDIGSQSTAPESIASNYFASERVVPESSTLPRQAFDLNSQIR
SQFPTLTQMLGDYPLCYLDTAATSQKPQCVLDAMAQYYLHDNANVHRAAHQLSARATSSY
ENVRDQLQLFINAKRREEIIFTHGTTESINLVAFGLTPQLKSGDLILIDSAAHHANIVPW
QELAKRTGAIIKPIPLTQDCRLDLNAYQALLELKPKVVALCHVSNALGTVNPVVELVRQA
KAQGALTLVDGAQAVAHLSVDMAAIDCDFYVFSGHKMYGPTGIGVLYGRFEILDTLTPLL
TGGEMIKRVSFDGTEFGSLPNRLEAGTPPISAVIGLGAAIRFLQLSLTLEVKAHEAELLQ
YLQNQLRALGDVHLYGAHSDNIGAIAFNLADEHHQDVGILLDQQGVAVRCGHHCAMPLMQ
TLNLKGCCRASIGIYTNKTDIDRFIAALASVKELLL