Protein Info for SO3774 in Shewanella oneidensis MR-1
Updated annotation (from data): proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.5.2; EC 1.2.1.88)
Rationale: Specific phenotype: utilization of Gelatin (gelatin contains a lot of proline)
Original annotation: proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase, putative (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 74% identity to fbl:Fbal_0550)Predicted SEED Role
"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88)" in subsystem Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.2.1.88, EC 1.5.99.8)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-proline degradation I (3/3 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-Nδ-acetylornithine biosynthesis (5/7 steps found)
- L-arginine degradation I (arginase pathway) (2/3 steps found)
- proline to cytochrome bo oxidase electron transfer (1/2 steps found)
- ethene biosynthesis II (microbes) (1/4 steps found)
- (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8EAW7 at UniProt or InterPro
Protein Sequence (1059 amino acids)
>SO3774 proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.5.2; EC 1.2.1.88) (Shewanella oneidensis MR-1) MFKASEVLAGRYDSANLDELFKAVTDNYIVDEEQYLSELIKLVPSSDEAIERVTRRAHEL VNKVRQFEKKGLMVGIDAFLQQYSLETQEGIILMCLAEALLRIPDAATADALIEDKLSGA KWDEHLSKSDSVLVNASTWGLMLTGKIVSLDKKIDGSSSNLLGRLVNRLGEPVIRQAMMA AMKIMGKQFVLGRTMKEALKNSEDKRKLGYTHSYDMLGEAALTRKDAEKYFTDYANAITE LGAQSYNESESPRPTISIKLSALHPRYEVANEDRVLTELYDTVIRLIKLARGLNIGISID AEEVDRLELSLKLFQKLFNSEATKGWGLLGIVVQAYSKRALPVLVWLTRLAKEQGDEIPV RLVKGAYWDSELKWAQQAGEAAYPLYTRKAGTDVSYLACARYLLSDATRGAIYPQFASHN AQTVAAISDMAGDRNHEFQRLHGMGQELYDTILSEAGAKAVRIYAPIGAHKDLLPYLVRR LLENGANTSFVHKLVDPKTPIESLVVHPLKTLTSYKTLANNKIVLPIDIFGSDRKNSKGL NMNIISEAEPFFAALDKFKSTQWQAGPLVNGQPLTGEHKTIVSPFDTTQTVGQVAFADKA AIEQAVSSAHAAFGSWTRTPVEVRASALQKLADLLEENREELIALCTREAGKSIQDGIDE VREAVDFCRYYAVQAKKLMSKPELLPGPTGELNELFLQGRGVFVCISPWNFPLAIFLGQV SAALAAGNTVVAKPAEQTSIIGYRAVQLAHQAGIPTDVLQYLPGTGATVGNALTADERIG GVCFTGSTGTAKLINRTLANREGAIIPLIAETGGQNAMVVDSTSQPEQVVNDVVSSSFTS AGQRCSALRVLFLQEDIADRVIDVLQGAMDELVIGNPSSIKTDVGPVIDATAKANLDAHI DHIKQVGKLIKQMSLPAGTENGHFVAPTAVEIDSIKVLEKEHFGPILHVIRYKASELAHV IDEINSTGFGLTLGIHSRNEGHALEVADKVNVGNVYINRNQIGAVVGVQPFGGQGLSGTG PKAGGPHYLTRFVTEKTRTNNITAIGGNATLLSLGDSDA