Protein Info for SO3741 in Shewanella oneidensis MR-1

Name: pntB
Annotation: NAD(P) transhydrogenase, beta subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 33 to 50 (18 residues), see Phobius details amino acids 56 to 76 (21 residues), see Phobius details amino acids 87 to 107 (21 residues), see Phobius details amino acids 129 to 153 (25 residues), see Phobius details amino acids 173 to 190 (18 residues), see Phobius details amino acids 196 to 214 (19 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 247 to 267 (21 residues), see Phobius details PF02233: PNTB" amino acids 7 to 467 (461 residues), 673.9 bits, see alignment E=6.4e-207

Best Hits

Swiss-Prot: 79% identical to PNTB_ECOL6: NAD(P) transhydrogenase subunit beta (pntB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 100% identity to son:SO_3741)

MetaCyc: 79% identical to pyridine nucleotide transhydrogenase subunit beta (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-277 [EC: 7.1.1.1]

Predicted SEED Role

"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2 or 7.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EAZ6 at UniProt or InterPro

Protein Sequence (469 amino acids)

>SO3741 NAD(P) transhydrogenase, beta subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MSQGLVTAAYIIAAVLFIFSLAGLSKQETAKHGNLFGITGMAIALLATIFNPDTSGVAWI
IVAMVIGGAIGVRLALKVEMTEMPELVAVLHSFVGMAAVLVGFNSFIDLHPEQVSQVGVG
AHMTGAMLNIHLVEVFLGIFIGAVTFTGSIVAFGKLRGLISSKPLMLPHRHKLNLIAVLV
SLGLLVYFVQTGGTMPALLLMTLIAFAFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAG
FMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGVASSSDQEMGEYRE
TNAEDVADMLKNATSVIITPGYGMAVAQAQYPVAEITQKLRDRGVKVRFGIHPVAGRLPG
HMNVLLAEAKVPYDIVLEMDEINEDFSDTDVVLVIGANDTVNPAAMEDPGSPIAGMPVLE
VWKAQNVIGFKRSMNTGYAGVQNPLFFKDNTQMLFGDAKASVEAILKAL