Protein Info for SO3664 in Shewanella oneidensis MR-1

Name: fadD-2
Annotation: long-chain-fatty-acid--CoA ligase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 22 to 395 (374 residues), 288 bits, E=1e-89
• PF13193: AMP-binding_C
  • amino acids 446 to 524 (79 residues), 78.2 bits, E=7.4e-26

Best Hits

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 100% identity to son:SO_3664)

Predicted SEED Role

"Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)" in subsystem Biotin biosynthesis or n-Phenylalkanoic acid degradation (EC 6.2.1.3)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• oleate β-oxidation (32/35 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of fatty acid biosynthesis II (plant) (37/43 steps found)
• fatty acid salvage (6/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• long-chain fatty acid activation (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• phosphatidylcholine acyl editing (2/4 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• octane oxidation (2/5 steps found)
• 2-deoxy-D-ribose degradation II (4/8 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (5/18 steps found)
• cutin biosynthesis (2/16 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.3

Sequence Analysis Tools

See Q8EB70 at UniProt or InterPro

Protein Sequence (532 amino acids)

>SO3664 long-chain-fatty-acid--CoA ligase (NCBI ptt file) (Shewanella oneidensis MR-1)
MAYDQESQLELGKYSSLIDLIERTSQRFGDKTAYACLGKNSSFNEIERDSRYFAAYLQNN
TNLKPGDRIAIQLPNITQFVIAAYGALRAGLILVNTNPLYTERELIHQFNDSGAKALVVL
SDLLPTLAKVVATTPIELVISTHPLDLIDPQVQPKTGLKNVEFCHVLQQGALLPFTRFVP
TLSDLAALQYTGGTTGLSKGAMLTHGNMLANAAQVKSRIGSVISEGEDIFVAPLPIYHIY
AFMVNLVLYFECGGCSVLIPNPRDINGLIKTLAKYPFTGFAGLNTLFVALCHQPEFKALN
FSHLKITISGGTALTAAAANLWQQTTGNTISEGYGLSETSPVISLNAPGYQKIGTIGKPV
LGTEVKLLDESGNEVTQGTAGELAARGPQVMLGYWNNPQETANVMTPDGFFKTGDIAILN
EEGFHQIVDRKKDMIIVSGFNVYPNEVENVLASHPNIIECAVVGVKDDHSGEAVKAFIVL
KDDSQDHEKIKTAILNFCREQLTAYKLPKQIEFMSQLPKSTVGKILRRELKN