Protein Info for SO3533 in Shewanella oneidensis MR-1

Name: ribF
Annotation: riboflavin biosynthesis protein RibF (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 PF06574: FAD_syn" amino acids 13 to 167 (155 residues), 189 bits, see alignment E=7.4e-60 TIGR00083: riboflavin biosynthesis protein RibF" amino acids 18 to 306 (289 residues), 340.1 bits, see alignment E=5.7e-106 PF01467: CTP_transf_like" amino acids 19 to 170 (152 residues), 24.9 bits, see alignment E=3.1e-09 PF01687: Flavokinase" amino acids 184 to 306 (123 residues), 149.3 bits, see alignment E=9e-48

Best Hits

Swiss-Prot: 56% identical to RIBF_ECOLI: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli (strain K12)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to son:SO_3533)

MetaCyc: 56% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBI3 at UniProt or InterPro

Protein Sequence (311 amino acids)

>SO3533 riboflavin biosynthesis protein RibF (NCBI ptt file) (Shewanella oneidensis MR-1)
MELIRGIHNILSSHHGCVLTIGNFDGVHRGHAQVIANLVQKAKQLSLPPTVMTFEPQPQE
LFRGENAPARLSLLRDKIVLLDELGVERLVCVNFNRAFADQPAEHFIEELLVRKLGIKYL
VVGDDFCFGKGRKGDFDMLKQAGTRYGFTVVSTQSFIVGSQRVSSTAVREQLAKGNLEQA
RRLLGHPFTLSGRVAHGQKIGRTIGFPTANIALKRNVVPVRGVFAVKLYWEDSDIYEGVA
NIGFRPTVNGQICQLEVHLLDFEGDIYGKHVEVELVAKLRDEQPFDSLDALKQQILNDAN
EARALFGNDAG