Protein Info for SO3532 in Shewanella oneidensis MR-1

Name: ileS
Annotation: isoleucyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 TIGR00392: isoleucine--tRNA ligase" amino acids 14 to 846 (833 residues), 990.2 bits, see alignment E=4.7e-302 PF00133: tRNA-synt_1" amino acids 28 to 643 (616 residues), 518.8 bits, see alignment E=3.5e-159 PF09334: tRNA-synt_1g" amino acids 58 to 201 (144 residues), 43 bits, see alignment E=9.5e-15 PF13603: tRNA-synt_1_2" amino acids 219 to 361 (143 residues), 28.7 bits, see alignment E=3.1e-10 PF08264: Anticodon_1" amino acids 688 to 844 (157 residues), 102.9 bits, see alignment E=5.4e-33 PF06827: zf-FPG_IleRS" amino acids 901 to 928 (28 residues), 30.8 bits, see alignment (E = 7.2e-11)

Best Hits

Swiss-Prot: 70% identical to SYI_AERS4: Isoleucine--tRNA ligase (ileS) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 70% identity to asa:ASA_0684)

MetaCyc: 70% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBI4 at UniProt or InterPro

Protein Sequence (940 amino acids)

>SO3532 isoleucyl-tRNA synthetase (NCBI ptt file) (Shewanella oneidensis MR-1)
MSDYKFTLNLPETEFPMRGNLANREPEMLERWTKDGLYQQIRDSRIGRTPFILHDGPPYA
NGSIHIGHSVNKILKDIIVKSKTMSGFDAPYVPGWDCHGLPIELKVEQKVGKPGQKISAA
EFREECRKYAAEQVDGQREDFIRLGVLGDWQNPYLTMDFATEANIVRSLSKVIENGHLHK
GVKPVHWCTDCGSALAEAEVEYEDKTSPAIDVAFTAVDSKAVAVKFGVSDYSHSVSMVIW
TTTPWTLPANRALSISPELDYSLVEFVKDGVTHAVILADVLVEACMTRYGAESHSVLAKI
KGAALELVRFKHPFLAFDVPAILGDHVTTDAGTGVVHTAPGHGQDDFVVGQKYGLEVANP
VGDNGVYKPDTEFFAGQHVFKANDNVVALLKEKGALLHHVAYRHSYPHCWRHKTPIIFRA
TPQWFISMDNHNLRKQALSEIEQIQWIPDWGQSRIEKMVENRPDWCISRQRTWGVPITLF
VHRETEELHPDSVSLMARVANRIEQEGIQAWWDLDAAELLGEEAEQYRKVTDTLDVWYDS
GSTFASVVAARPEFHGHGVDLYLEGSDQHRGWFMSSLMISTAMTGKAPYKQVLTHGFTVD
GKGRKMSKSIGNVIAPQQVTNKLGADILRLWVAATDYSGEMTVSDEILNRSADAYRRIRN
TARFLLANLNGFDPKNDLVAVEDMVALDRWAVRRAAALQQEIIEAYEQYNFHIVTQKLMQ
FCSIELGSFYLDIIKDRQYTAKQEGHARRSCQSALFHIAEAMVRWIAPVLSFTADEVWQL
LPGQRDAYVFTQEWYQGLQSITLDTDLSDAYWENLLTVRNEVNKVIEQARRDKRVGGSLE
AEVTLFADATLTEQLTHIGDELRFVLLTSEAKVLPLVDATSDAVETELASLKLVVNATTA
EKCERCWHHREEVGTIEAHPTLCHRCVTNIEGDGEVRLFA