Protein Info for SO3471 in Shewanella oneidensis MR-1

Name: glyA
Annotation: serine hydroxymethyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 9 to 386 (378 residues), 595.5 bits, E=4e-183
• PF00155: Aminotran_1_2
  • amino acids 80 to 322 (243 residues), 34.5 bits, E=1.4e-12

Best Hits

Swiss-Prot: 100% identical to GLYA_SHEON: Serine hydroxymethyltransferase (glyA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 100% identity to son:SO_3471)

MetaCyc: 83% identical to serine hydroxymethyltransferase (Escherichia coli K-12 substr. MG1655)
4.1.2.-; Glycine hydroxymethyltransferase. [EC: 2.1.2.1]; RXN-6321 [EC: 2.1.2.1]; RXN0-5240 [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations II (plants) (11/11 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• photorespiration II (8/10 steps found)
• folate transformations I (10/13 steps found)
• photorespiration I (7/9 steps found)
• photorespiration III (7/9 steps found)
• glycine biosynthesis I (1/1 steps found)
• glycine betaine degradation III (4/7 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• glycine betaine degradation I (4/8 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See Q8EBN8 at UniProt or InterPro

Protein Sequence (417 amino acids)

>SO3471 serine hydroxymethyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
LAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
VDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAADDEELYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKTYQQQVVNNAKAMVE
VFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
GVRIGTPAITRRGFKEAEAKQLTGWICDILDDAHNPAVIERVKGQVLALCARFPVYG