Protein Info for SO_3457 in Shewanella oneidensis MR-1

Annotation: hybrid sensory histidine kinase BarA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 935 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 184 to 205 (22 residues), see Phobius details PF09984: sCache_4" amino acids 37 to 184 (148 residues), 181.9 bits, see alignment E=2.2e-57 PF00672: HAMP" amino acids 206 to 256 (51 residues), 33.1 bits, see alignment 2e-11 PF00512: HisKA" amino acids 301 to 365 (65 residues), 74.4 bits, see alignment 2.1e-24 PF02518: HATPase_c" amino acids 412 to 526 (115 residues), 91.3 bits, see alignment E=2e-29 PF00072: Response_reg" amino acids 684 to 795 (112 residues), 90.5 bits, see alignment E=2.9e-29 PF01627: Hpt" amino acids 830 to 913 (84 residues), 59 bits, see alignment E=1.6e-19

Best Hits

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 100% identity to son:SO_3457)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBQ2 at UniProt or InterPro

Protein Sequence (935 amino acids)

>SO_3457 hybrid sensory histidine kinase BarA (RefSeq) (Shewanella oneidensis MR-1)
MNPVNNMTKYSLRSWVLVLALAPTILVGILLGSYFTINRFYELEDTLIEQGSNIIEPLAI
ASEVGLVGNDREATKRLLAAAQLNKSTLVKSIAIFDIHNQLFVTSHYHKDFEIMRYKEAL
TNLQKTEIEHVGDSLILRTPIFATATPTGSEPANVDIQTDNGELLGYISVIINKERALLE
QHRAAVAAFIIVLIGVQLNLLFTFRLVKNVTQPITEMVRVVAKIREGKLDARLQGNLIGE
LDLLKRGINAMAGSLSEYHDEMQQNIDQATSDLRETLEQIEIQNVELDLAKKRALEASRI
KSEFLANMSHELRTPLNGVIGFARQLVKTPLHSSQVDYINTIERSATNLLAIINDILDFS
KLEAGKMVLESMPFGLRETLGETITLISGSAQAKGLELVVDIAPNVPDNVNGDSMRVCQI
INNLVGNAIKFTDSGSVLVKLELQAQTDEQVVLRCDVIDTGIGIDESQQDFLFQAFGQAD
SSISRRFGGTGLGLVITKRLVNQMGGQIGFTSSVDKGSNFWFTLPLGLGQFQIGDSLPLE
KLQNKTVLFYEPRVLTHSVISRQLKQWDTKVTHHQHIPSLQTTLSTTEHQFDYVLLSCHG
FNNPNHLVSTLIQAKAKTDCLIVLFDCQEQDMLSQFIRPNADVVLSLPVSEHQLARNMLY
PPMEYDLLPPVSIAVPAARQSLTVLAVDDNFANLKLIDTLLNELVTTVIAVNSGEEAVKQ
AKTRTFDLIFMDIQMPGTDGISATKQIRQGSMNRNTPIIAVTAHAIAEERELILGSGMDG
YLPKPIDEAALKAEINRWITRPKFTHFDLHTLNWDLCLTQANHKSGLALDMLRMLLDSLP
QTVSAIEAALSQNDQVTMLSTIHKLHGASCYCGVPTTQRLCQEIESALKREARVEDLEPE
ILELLDELTKVESAAKQVLSQLSAEITDDQKNGLL