Protein Info for SO3455 in Shewanella oneidensis MR-1

Name: relA
Annotation: GTP pyrophosphokinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 PF13328: HD_4" amino acids 47 to 190 (144 residues), 136.5 bits, see alignment E=2.1e-43 TIGR00691: RelA/SpoT family protein" amino acids 63 to 733 (671 residues), 621.1 bits, see alignment E=1.2e-190 PF04607: RelA_SpoT" amino acids 250 to 360 (111 residues), 146.9 bits, see alignment E=8e-47 PF02824: TGS" amino acids 404 to 463 (60 residues), 67.4 bits, see alignment 2.6e-22 PF19296: RelA_AH_RIS" amino acids 483 to 636 (154 residues), 47.1 bits, see alignment E=9.6e-16 PF13291: ACT_4" amino acids 658 to 734 (77 residues), 58.9 bits, see alignment E=1.9e-19

Best Hits

Swiss-Prot: 62% identical to RELA_ECOL6: GTP pyrophosphokinase (relA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to son:SO_3455)

MetaCyc: 62% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBQ4 at UniProt or InterPro

Protein Sequence (735 amino acids)

>SO3455 GTP pyrophosphokinase (NCBI ptt file) (Shewanella oneidensis MR-1)
MVSVREAHFNDPDFHLEEWVARYVSHVDEAQTLLALIAQVDALPAKSPAAKRELLEHARE
MIEILAPLNMDIETLQAAILFVVFDAGLLNEEAIKEKFGEPLARLVASVVTMNAIGALKI
NPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLRAVKNADEETRVLLAREIADI
YAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIENFVEQLQQRLD
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWH
HIPREFDDYVANPKPNGYQSIHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGN
HSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVFEDRVYVFTPSGEVVDLPLGS
TVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
IRTSRARSKIQHWFKQQDRDKNIIAGKEMLEAELARVSLRIKDATIAVERFNMASMDDLL
AAIGGGDVRLHQVVNHIQSKLRLDEASEEDAVEELVKKSQSKSGTNSRGQVEVNGVGNLL
SHIARCCQPVPGDEILGFITKGRGISVHRSDCEQVKELMRVHPERGVDVVWGENYSGGYR
MRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDIKNQTAAIELELELYNLDGLSRVL
SKLSQVDSVIEARRL