Protein Info for SO3434 in Shewanella oneidensis MR-1

Name: pcm
Annotation: protein-L-isoaspartate O-methyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 PF01135: PCMT" amino acids 11 to 209 (199 residues), 224.4 bits, see alignment E=7.2e-71 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 12 to 210 (199 residues), 246.6 bits, see alignment E=1.2e-77

Best Hits

Swiss-Prot: 100% identical to PIMT_SHEON: Protein-L-isoaspartate O-methyltransferase (pcm) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to son:SO_3434)

MetaCyc: 60% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBR6 at UniProt or InterPro

Protein Sequence (211 amino acids)

>SO3434 protein-L-isoaspartate O-methyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTRVALTSAVNLAKKLQDAGIRNPAVLKAISQTPRELFLDNALAHKAYENTALPIGQGQT
ISQPYIVARMTELLLQHQPQKVLEVGTGSGYQAAILAQLVPELCTVERIKALQIQARQRL
KRLDLHNVSFKYGDGWQGWLNRSPYNGIMVTAAAAKIPEALLSQLAEGGVLIIPVGEETQ
QLMRITRHSDRFSSEVIETVKFVPLVNGELA