Protein Info for SO3424 in Shewanella oneidensis MR-1

Name: valS
Annotation: valyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 transmembrane" amino acids 504 to 524 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 936 (936 residues), 1236.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 631 (618 residues), 530.3 bits, see alignment E=7.7e-163 PF09334: tRNA-synt_1g" amino acids 40 to 189 (150 residues), 29.5 bits, see alignment E=8.3e-11 PF13603: tRNA-synt_1_2" amino acids 246 to 323 (78 residues), 37 bits, see alignment E=6.3e-13 PF08264: Anticodon_1" amino acids 679 to 829 (151 residues), 145.1 bits, see alignment E=3.7e-46 PF10458: Val_tRNA-synt_C" amino acids 889 to 949 (61 residues), 70.9 bits, see alignment 2.2e-23

Best Hits

Swiss-Prot: 72% identical to SYV_ECO57: Valine--tRNA ligase (valS) from Escherichia coli O157:H7

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 72% identity to aha:AHA_3702)

MetaCyc: 72% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBS5 at UniProt or InterPro

Protein Sequence (958 amino acids)

>SO3424 valyl-tRNA synthetase (NCBI ptt file) (Shewanella oneidensis MR-1)
MEKTYDPQSIEQTLYQNWEEKGYFKPHGDASQGNYCIMIPPPNVTGSLHMGHAFQDTIMD
TLIRYQRMKGKNTLWQVGTDHAGIATQMLVERKLEAEEGKSRHDLGRDVFMEKVWEWKAQ
SGGTITKQLRRMGASVDWDRERFTMDEGLSKAVQEVFVRLYEDDLIYRGKRLVNWDPKLH
TAISDLEVENKEKQGHMWHLRYPLADGELTADGKDYLEVATTRPETMLGDSAVAVHPDDE
RYQALIGKYILLPIVNRRIPIVADDYVDMAFGTGCVKITPAHDFNDYEVGKRHKLPMFNV
LTLDAAIRSSAEVVNTDGTINTSLDGSLPERYAGLDRFKARDAIVAEFETLGLLEKIAPH
GLKVPYGDRSGVVIEPMLTDQWYVAVAPMAKTAIEAVENGDIKFVPQQYENMYFSWMRDI
QDWCISRQLWWGHRIPAWYDTNGKVYVGRTEAEVRAKHDIDDAIALRQDEDVLDTWFSSA
LWTFSTLGWPDNVEDLKTFHPTDVLVTGFDIIFFWVARMIMMTMHFIKDEDGKPQVPFKT
VYVTGLIRDEAGNKMSKSKGNVLDPLDMIDGIDLETLVEKRTGNMMQPQLAAKIEKSTRK
EFENGIEAHGTDALRFTLAAMASTGRDINWDMKRLDGYRSFCNKLWNASRYVLMNTEGQD
CGPNSPDYQGGEMELSLADRWIIDLFNQTVKTYDEHMASYRFDLAANTLYEFTWNQFCDW
YLELTKPVLQNGTEAQMRGTRHTLVNVLEAMQRLMHPMMPYITETIWQRVKPLTGVQGDT
IMLAPFPAFDAAKVDATAMADLEWVKQVIVAVRNIRAELNIAPSKPLNALLRGVSAQDKA
RIEANQAFFATLARLESMTILGEGETAPMSTTGLIGEMELLIPMAGLVDVAAEMARIDKQ
LEKLTQEIARIEGKLSNEGFVAKAPPAVIDKERAKMADLSRDIDKLTEQKAEFAKLEA