Protein Info for SO3414 in Shewanella oneidensis MR-1

Name: thrB
Annotation: homoserine kinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00191: homoserine kinase
  • amino acids 3 to 309 (307 residues), 291.5 bits, E=2.7e-91
• PF00288: GHMP_kinases_N
  • amino acids 87 to 153 (67 residues), 61 bits, E=1.2e-20
• PF08544: GHMP_kinases_C
  • amino acids 235 to 285 (51 residues), 30.5 bits, 4e-11

Best Hits

Swiss-Prot: 55% identical to KHSE_VIBC3: Homoserine kinase (thrB) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K00872, homoserine kinase [EC: 2.7.1.39] (inferred from 100% identity to son:SO_3414)

MetaCyc: 56% identical to homoserine kinase (Escherichia coli K-12 substr. MG1655)
Homoserine kinase. [EC: 2.7.1.39]; 2.7.1.- [EC: 2.7.1.39]

Predicted SEED Role

"Homoserine kinase (EC 2.7.1.39)" in subsystem Methionine Biosynthesis or Threonine and Homoserine Biosynthesis (EC 2.7.1.39)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-threonine biosynthesis (2/2 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.39

Sequence Analysis Tools

See Q8EBT5 at UniProt or InterPro

Protein Sequence (312 amino acids)

>SO3414 homoserine kinase (NCBI ptt file) (Shewanella oneidensis MR-1)
MSLTVYAPASMGNVGVGFDLLGAALAPIDGSLLGDRVTIAAADGGVSLTQVGGWAHKLPS
NPEDNIVHQCAVFFLKALGKENRGVALTLEKNLPVGSGLGSSASSVVAALYALNEHFGCP
YDKQALLELMGEFEGKISGSVHYDNVAPCYLGGMQLMLNVPGAICEAIPSFKQWYWVVAY
PGISLSTAMMRKLMPEQYDKSVVIDFGRYLSAFVHASYQQNAQLAIQVLKDVLAEPYRAA
AIPGYDNARAALAELGMLTTGISGSGPTLFSVTDDLATANAAKAWLEANYLTDAGGFAHV
CRLDEQGTRRVD