Protein Info for SO3088 in Shewanella oneidensis MR-1

Annotation: fatty oxidation complex, alpha subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 707 TIGR02440: fatty oxidation complex, alpha subunit FadJ" amino acids 6 to 707 (702 residues), 1336.6 bits, see alignment E=0 PF00378: ECH_1" amino acids 12 to 216 (205 residues), 140.8 bits, see alignment E=9.9e-45 PF16113: ECH_2" amino acids 18 to 215 (198 residues), 89.9 bits, see alignment E=4.8e-29 PF02737: 3HCDH_N" amino acids 315 to 493 (179 residues), 188.4 bits, see alignment E=2.3e-59 PF00725: 3HCDH" amino acids 496 to 589 (94 residues), 90.2 bits, see alignment E=2e-29 amino acids 619 to 702 (84 residues), 34.2 bits, see alignment E=6.1e-12

Best Hits

Swiss-Prot: 100% identical to FADJ_SHEON: Fatty acid oxidation complex subunit alpha (fadJ) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 100% identity to son:SO_3088)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8ECP7 at UniProt or InterPro

Protein Sequence (707 amino acids)

>SO3088 fatty oxidation complex, alpha subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MAVGKTFNLTRREDGIAILTMDVPGETMNTLKAEFGPEISEILSEIKRDSSIRGLVLISG
KKDSFVAGADISMLDACQTAGDAKALSQQGHVVFNELEALNIPVVAAIHGACLGGGLELA
LACHQRVCSDDGKTMLGVPEVQLGLLPGGGGTQRLPRLVGITTALDMMLTGKQIRPKQAL
KMGLVNDVVPQTILLQTAVEMALAGKQIAKPVKKSLVNQLLEGTGFGRNIIFDQAAKQVA
KKTQGNYPAPAKIIDCVRQGIAKGMQKGLEVEASHFAELVVSKESEALRSIFFATTEMKK
ETGAEGATPRKVKKAVILGGGLMGGGIASVTTTKAKIPARVKDINEKGLSNALSYAYKLL
DKGVKRRHMTPAARDNLMALMTTTTEYKGVKDADIVVEAVFEDLALKHQMVKDIERECGE
HTIFASNTSSLPIGQIAQAASRPENVIGLHYFSPVEKMPLVEVIAHAKTSPETIATTVAF
ARKQGKTPIVVQDGAGFYVNRILALYMNEAAQLLLEGQSVEHLDKALVKFGFPVGPITLL
DEVGIDVGAKISPILEKELGERFKAPAAFDKLLSDDRKGRKNGKGFYQYGAASKKKAVDE
TVYGVLGIKPGVDKEMSAVAERCVVQMLNEAVRCLDAGIIASPRDGDIGAIFGIGFPPFL
GGPFHYIDTLGADNLVKILERYQTQYGDRFEPCQRLKAMAAEKARFF