Protein Info for SO2882 in Shewanella oneidensis MR-1

Updated annotation (from data): yeaG component of nitrogen-related signalling system (of yeaGH-ycgB)
Rationale: conserved cofitness; yeaG is a protein kinase
Original annotation: hypothetical Ser protein kinas (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 PF08298: AAA_PrkA" amino acids 20 to 380 (361 residues), 584.3 bits, see alignment E=9.4e-180 PF06798: PrkA" amino acids 382 to 635 (254 residues), 225.7 bits, see alignment E=8.4e-71

Best Hits

Swiss-Prot: 84% identical to YEAG_ECOLI: Uncharacterized protein YeaG (yeaG) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 84% identity to eae:EAE_21735)

MetaCyc: 84% identical to protein kinase YeaG (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

"Serine protein kinase (prkA protein), P-loop containing"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8ED82 at UniProt or InterPro

Protein Sequence (644 amino acids)

>SO2882 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (Shewanella oneidensis MR-1)
MGIFEHYQKRYEQKLDEEYTLQEFLDICKEDRSAYMSASERLLLAIGEAEIIDTAKNPTL
SRIFSNRLISRYPAFKDFFGMEDAIEQIVAYLKHSAQGLEESKQILYLLGPVGGGKSSLA
EKLKSLMQKVPIYILSANGVRSPVNDHPFCLFDVDEDGQLLRDEFNIPSRYLKTIMSPWA
VKRLHEFGGDISKFRVVKVYPSILDQLAIAKTEPGDENNQDISSLVGKVDIRQLEHYSQD
DADAYAYSGALCRANQGIMEFVEMFKAPIKVLHPLLTATQEGNYNGTEGLSALPYSGIIL
AHSNESEWSTFKNNKNNEAFLDRVYIVKVPYCLRVSEELQIYKKLIQNSELSQAPCAPGT
IETLAQFSVLSRIKAPENSSIYSKMRVYDGESLKDTDPKAKSYQEYRDYAGVDEGMNGLS
TRFAFKILSRVFNFDHSEIAANPVHLFYVLERQIEQEQFPGDTAERYLEFLKGYLIPKYV
EFIGKEIQTAYLESYSEYGQNIFDRYVTYADFWIQDQEYRDPETGQLFDRAALNAELEKI
EKPAGISNPKDFRNEIVNFVLRARASHEGHNPLWTSYEKLRTVIEKKMFSNTEDLLPVIS
FNAKTSNDDQRKHDDFVNRMMEKGYTKKQVRLLSEWYLRVRKSS