Protein Info for SO2802 in Shewanella oneidensis MR-1

Name: msbA
Annotation: ABC transporter, ATP-binding protein MsbA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 signal peptide" amino acids 25 to 25 (1 residues), see Phobius details transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 82 to 109 (28 residues), see Phobius details amino acids 159 to 178 (20 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details amino acids 263 to 287 (25 residues), see Phobius details amino acids 295 to 313 (19 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 13 to 600 (588 residues), 747.3 bits, see alignment E=5.4e-229 PF00664: ABC_membrane" amino acids 28 to 315 (288 residues), 183 bits, see alignment E=9.9e-58 PF00005: ABC_tran" amino acids 377 to 528 (152 residues), 107.2 bits, see alignment E=1.1e-34

Best Hits

Swiss-Prot: 100% identical to MSBA_SHEON: Lipid A export ATP-binding/permease protein MsbA (msbA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 100% identity to son:SO_2802)

MetaCyc: 57% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA (EC 3.6.3.25)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 3.6.3.25)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.25

Use Curated BLAST to search for 3.6.3.- or 3.6.3.25 or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EDF0 at UniProt or InterPro

Protein Sequence (601 amino acids)

>SO2802 ABC transporter, ATP-binding protein MsbA (NCBI ptt file) (Shewanella oneidensis MR-1)
MTASPKNEMWTVFKRLMGYLKPMKGMFLLSVVGLIVYGLVDAAFISFIGPFIDKGFSSSA
PAISNGIALPTSQGFHADNQVLLMAPIVVILMFSLRGFANFVSTYGISYMSARLIMDMRQ
QVFEHYLSLPVSYMDKENTGNLISKVTFDTEQIARASGSALISIVRDSVTIIGMLGLMFY
NSWKLSLCILVIGPIMGVVITIVSRRFRKVSKQIQTAMGDVSAATEQMIKGHKNVLAFGG
QETETARFAKINDRNRHQNMKLAVAQAVSQPLIMVIGSFALAFVLYAASLDSMKADLTAG
TFATILGAMMAMLQPIKNLTRVNAEFQRGIAACTTVFELLDTVPESDTGTYTVARAKGNL
RFDNVSFSYEGQERRALEKIDFEVSQGQTLALVGRSGSGKSTIASLVTRFYTGLESGDIL
LDDVSIYDYSLKSLRSQVALVSQQVTLFNDTIANNIAYAYPGEVTREQIIEAATLAHAME
FIEQLPDGLDTQVGENGVLLSGGQRQRIAIARAMLRDAPVLILDEATSALDTESEKAIQQ
GLDNLRQNRTSVVIAHRLSTIESADQILVVDQGRIVERGTHKSLLELGGMYAKLYQMQFG
S