Protein Info for SO2706 in Shewanella oneidensis MR-1

Name: astB
Updated annotation (from data): Succinylarginine dihydrolase (EC 3.5.3.23)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCARGDIHYDRO-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: succinylarginine dihydrolase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04996: AstB
  • amino acids 3 to 443 (441 residues), 739.1 bits, E=8e-227
• TIGR03241: succinylarginine dihydrolase
  • amino acids 4 to 443 (440 residues), 733.2 bits, E=6.2e-225

Best Hits

Swiss-Prot: 100% identical to ASTB_SHEON: N-succinylarginine dihydrolase (astB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01484, succinylarginine dihydrolase [EC: 3.5.3.23] (inferred from 100% identity to son:SO_2706)

MetaCyc: 54% identical to N-succinylarginine dihydrolase (Escherichia coli K-12 substr. MG1655)
N-succinylarginine dihydrolase. [EC: 3.5.3.23]

Predicted SEED Role

"Succinylarginine dihydrolase (EC 3.5.3.23)" in subsystem Arginine and Ornithine Degradation (EC 3.5.3.23)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.3.23

Sequence Analysis Tools

See Q8EDN7 at UniProt or InterPro

Protein Sequence (444 amino acids)

>SO2706 Succinylarginine dihydrolase (EC 3.5.3.23) (Shewanella oneidensis MR-1)
MKHFEANFDGLVGPTHNYAGLSFGNVASLNNAALVSNPKAAAKQGLQKAKALADLGMIQG
MLAPQERPDLNTLRRIGFSGSDAQVLQQAAKTAPALLNACCSASSMWTANAATVSPSADT
RDGKLHFTPANLVDKLHRSIEPITTGRILTATFNDPHYFYHHNHLPEHNSFGDEGAANHT
RLCQEYGHAGVELFVYGQEATNPHAPKPLKFPARQTLEASMAIARLHQLEEDNCVFIQQN
PAVIDQGVFHNDVIAVGNQNVLFYHEQAFLNTQAKLDEIKRKLDTELYFIEVPTAKVSIN
DAVKSYLFNTQIITLPSGEMAIIAPTDCQENPAVYAYLNELLSLNTPIKQVLYFDVKQSM
QNGGGPACLRLRVAMNEREVAAVNQHTLLTDALFTRLNTWVEKHYRDRLSTEDLADPQLV
IESRTALDELTQIMKLGSVYPFQR