Protein Info for SO2633 in Shewanella oneidensis MR-1

Name: trmU
Annotation: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 PF02568: ThiI" amino acids 10 to 80 (71 residues), 31.1 bits, see alignment E=5.3e-11 PF02540: NAD_synthase" amino acids 10 to 80 (71 residues), 32.9 bits, see alignment E=1.1e-11 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 11 to 366 (356 residues), 507.8 bits, see alignment E=6.6e-157 PF03054: tRNA_Me_trans" amino acids 11 to 208 (198 residues), 282.1 bits, see alignment E=7.5e-88 PF20259: tRNA_Me_trans_M" amino acids 214 to 281 (68 residues), 80.9 bits, see alignment E=1.2e-26 PF20258: tRNA_Me_trans_C" amino acids 291 to 366 (76 residues), 86.8 bits, see alignment E=3.1e-28

Best Hits

Swiss-Prot: 100% identical to MNMA_SHEON: tRNA-specific 2-thiouridylase MnmA (mnmA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 100% identity to son:SO_2633)

MetaCyc: 76% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8CX40 at UniProt or InterPro

Protein Sequence (372 amino acids)

>SO2633 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTSIEPTHTGKKVIVGMSGGVDSSVSAYLLMQQGYQVEGLFMKNWEEDDNDEYCAAAEDL
KDAQAVCDKLGIKLHTVNFAAEYWDNVFEYFLAEYKAGRTPNPDIMCNKEIKFKAFLEFA
DDILDADYIAMGHYVRRRDNEDGTTQMLRGVDNNKDQSYFLYTLSHEQVARSLFPVGELE
KHEVREIAKQMGLITHDKKDSTGICFIGERKFTEFLGNYLPAQPGNIETADGEVIGTHQG
LMYHTLGQRKGLGIGGMKNSNDDPWYVVDKDLKRNVLVVGQGGHHPRLMSNGMLVNQLHW
VDRKGPINGSQIVVKTRYRQQDIPCTLTYLDDNTLKVVFDEPVAAVTPGQSAVFYDGEVC
LGGGIIDQLIRG